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StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity

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Science Advances  04 Nov 2016:
Vol. 2, no. 11, e1601266
DOI: 10.1126/sciadv.1601266

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Abstract

Strigolactones are key regulators of plant development and interaction with symbiotic fungi; however, quantitative tools for strigolactone signaling analysis are lacking. We introduce a genetically encoded hormone biosensor used to analyze strigolactone-mediated processes, including the study of the components involved in the hormone perception/signaling complex and the structural specificity and sensitivity of natural and synthetic strigolactones in Arabidopsis, providing quantitative insights into the stereoselectivity of strigolactone perception. Given the high specificity, sensitivity, dynamic range of activity, modular construction, ease of implementation, and wide applicability, the biosensor StrigoQuant will be useful in unraveling multiple levels of strigolactone metabolic and signaling networks.

Keywords
  • Strigolactone signaling
  • strigolactone quantitative biosensor
  • plant synthetic biology
  • genetically encoded strigolactone biosensor
  • strigolactone perception

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

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