Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions

Vasilios Kalas; Jerome S. Pinkner; Thomas J. Hannan; Michael E. Hibbing; Karen W. Dodson; Alex S. Holehouse; Hao Zhang; Niraj H. Tolia; Michael L. Gross; Rohit V. Pappu; James Janetka; Scott J. Hultgren

doi:10.1126/sciadv.1601944

Research ArticleBIOMOLECULES

Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions

Vasilios Kalas¹,²,³,
Jerome S. Pinkner¹,²,
Thomas J. Hannan¹,²,⁴,
Michael E. Hibbing¹,²,
Karen W. Dodson¹,²,
Alex S. Holehouse³,⁵,
Hao Zhang⁶,
Niraj H. Tolia²,
Michael L. Gross⁶,
Rohit V. Pappu³,⁵,
James Janetka⁶ and
Scott J. Hultgren¹,²,*

¹Center for Women’s Infectious Disease Research, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
²Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
³Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.
⁴Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
⁵Department of Biomedical Engineering and Center for Biological Systems Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
⁶Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.

↵*Corresponding author. Email: hultgren{at}wustl.edu

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Science Advances 10 Feb 2017:
Vol. 3, no. 2, e1601944
DOI: 10.1126/sciadv.1601944

Vasilios Kalas

Center for Women’s Infectious Disease Research, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.

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Jerome S. Pinkner

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Thomas J. Hannan

Center for Women’s Infectious Disease Research, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.

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Michael E. Hibbing

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Karen W. Dodson

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Alex S. Holehouse

Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Biomedical Engineering and Center for Biological Systems Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.

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Hao Zhang

Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.

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Niraj H. Tolia

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

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Michael L. Gross

Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.

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Rohit V. Pappu

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James Janetka

Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.

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Scott J. Hultgren

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For correspondence: hultgren@wustl.edu

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Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions

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