Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions

Vasilios Kalas; Jerome S. Pinkner; Thomas J. Hannan; Michael E. Hibbing; Karen W. Dodson; Alex S. Holehouse; Hao Zhang; Niraj H. Tolia; Michael L. Gross; Rohit V. Pappu; James Janetka; Scott J. Hultgren

doi:10.1126/sciadv.1601944

Research ArticleBIOMOLECULES

Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions

Vasilios Kalas¹,²,³,
Jerome S. Pinkner¹,²,
Thomas J. Hannan¹,²,⁴,
Michael E. Hibbing¹,²,
Karen W. Dodson¹,²,
Alex S. Holehouse³,⁵,
Hao Zhang⁶,
Niraj H. Tolia²,
Michael L. Gross⁶,
Rohit V. Pappu³,⁵,
James Janetka⁶ and
Scott J. Hultgren¹,²,*

¹Center for Women’s Infectious Disease Research, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
²Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
³Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.
⁴Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
⁵Department of Biomedical Engineering and Center for Biological Systems Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
⁶Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.

↵*Corresponding author. Email: hultgren{at}wustl.edu

See allHide authors and affiliations

Science Advances 10 Feb 2017:
Vol. 3, no. 2, e1601944
DOI: 10.1126/sciadv.1601944

Vasilios Kalas

Center for Women’s Infectious Disease Research, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Vasilios Kalas

Jerome S. Pinkner

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Jerome S. Pinkner

Thomas J. Hannan

Center for Women’s Infectious Disease Research, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Thomas J. Hannan

Michael E. Hibbing

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Michael E. Hibbing

Karen W. Dodson

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Alex S. Holehouse

Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.Department of Biomedical Engineering and Center for Biological Systems Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Alex S. Holehouse

Hao Zhang

Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Niraj H. Tolia

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Michael L. Gross

Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
ORCID record for Michael L. Gross

Rohit V. Pappu

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

James Janetka

Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site

Scott J. Hultgren

Find this author on Google Scholar
Find this author on PubMed
Search for this author on this site
For correspondence: hultgren@wustl.edu

Article
Figures & Data
Info & Metrics
eLetters
PDF

eLetters is an online forum for ongoing peer review. Submission of eLetters are open to all . Please read our guidelines before submitting your own eLetter.

Submit a Response to This Article

Compose eLetter

Title

Contents

More information about text formats

Plain text

Plain text
No HTML tags allowed.
Web page addresses and e-mail addresses turn into links automatically.
Lines and paragraphs break automatically.

Author Information

Contributors
First name and middle name First or given name, e.g. 'Peter'. Last Name Your last, or family, name, e.g. 'MacMoody'. Email Your email address, e.g. higgs-boson@gmail.com Role / Occupation Your role and/or occupation, e.g. 'Orthopedic Surgeon'. Affiliation Your organization or institution (if applicable), e.g. 'Royal Free Hospital'.

Statement of Competing Interests

Competing interests?

Yes

Please describe the competing interests

CAPTCHA

This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

What code is in the image? Enter the characters shown in the image.

Vertical Tabs

No eLetters have been published for this article.

View Full Text

Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions

Plain text

Vertical Tabs

Science Advances

Article Tools