Research ArticleCELL BIOLOGY

The role of electron irradiation history in liquid cell transmission electron microscopy

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Science Advances  20 Apr 2018:
Vol. 4, no. 4, eaaq1202
DOI: 10.1126/sciadv.aaq1202

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  • Correction for the role of electron irradiation history in transmission electron microscopy
    • Trevor Moser, Graduate Student, Michigan Technological University
    • Other Contributors:
      • James Evans, Scientist, Pacific Northwest National Laboratory
      • Tolou Shokuhfar, Associate Professor, University of Illinois in Chicago

    We request that readers consider two replacement sentences for our manuscript entitled “The role of electron irradiation history in liquid cell transmission electron microscopy” since two of the original sentences relayed different meaning to readers than intended.

    On page 4 of our publication we would like to replace the sentence “…although several studies have attempted to demonstrate the viability of cells irradiated with the electron beam by using so-called live/dead cell fluorescent assays (3, 36).” For clarity, we recognize a better version of that statement would be “although several studies have attempted to evaluate the viability of cells before and/or after irradiation with the electron beam by using so-called live/dead cell fluorescent assays (3, 31, 36).”
    On page 5, we make the statement “Other previous studies have attempted to draw physiological conclusions about cellular behavior based on tracking particle motion relative to the cell (32, 38).” In the interest of clarity, we note that this sentence should have read “Other previous studies have used nanoparticles to localize the position of labelled proteins within a chemically fixed cell (32,38). For future experiments repeating that work, the potential of particle motion from pre-irradiation should be accounted for and the flux highly controlled.” in order to better reflect the cited references.
    Overall, we hope that the science community accepts these suggested changes and we apologize...

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    Competing Interests: None declared.

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