Science Advances

Supplementary Materials

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  • Fig. S1. Two-color dSTORM super-resolution imaging of MyBP-C and ryanodine receptors (RyR2).
  • Fig. S2. Treatment with the contractile inhibitor blebbistatin does not obscure sarcomeric calcium gradients.
  • Fig. S3. Calcium-dependent regulation of native thin filament motion.
  • Fig. S4. Histograms for thin filaments on λ-phosphatase–treated thick filaments containing dephosphorylated MyBP-C (trajectory length ± SEM and percent trajectories with biphasic velocities versus pCa).
  • Fig. S5. 3D reconstructions of low-Ca2+ native thin filaments decorated with C0C3 (actin/C0C3,1:3) in which the four phosphorylatable serines have been replaced with alanines (fully dephosphorylated, C0C3-4A) or with aspartic acid residues (phosphomimetic, C0C3-4D).
  • Fig. S6. Transverse sections of the respective low-Ca2+ reconstructions in fig. S5, in which native thin filaments have been decorated with C0C3-4A and C0C3-4D.
  • Fig. S7. Phosphorylation of MyBP-C N-terminal domains modulates their ability to activate native thin filament motion in the in vitro motility assay on a bed of monomeric myosin.

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