Science Advances

Supplementary Materials

This PDF file includes:

  • Text
  • Table S1. Samples obtained from the 34 Amerindian subjects included in this study: skin from right (BD) or left (BI) forearm (n = 28), oral (n = 28), and fecal (n = 12) specimens.
  • Table S2. Composition of Amerindian E. coli isolate genomic libraries.
  • Table S3. Functional capture of AR genes from Amerindian E. coli isolate genomic libraries.
  • Table S4. AR genes identified from functional selections of Amerindian E. coli isolate genomic libraries and Amerindian and Puerto Rican metagenomic libraries.
  • Table S5. Composition of metagenomic libraries from Amerindian and Puerto Rican fecal and oral microbiota.
  • Table S6. Antibiotics used in genomic and metagenomic library selections for AR genes.
  • Table S10. List of clusters at different k-mer and minimum contig length and their associated taxonomy.
  • Table S11. Taxa of 329 bacterial strains cultured from feces of 12 isolated Amerindians.
  • Table S12. Typing of 24 E. coli strains from 11 isolated Amerindians.
  • Fig. S1. Meta-analysis of 16S V4 region fecal data.
  • Fig. S2. Microbiome diversity between different human groups.
  • Fig. S3. Microbiota diversity in fecal, oral, and skin samples from uncontacted Yanomami in relation to U.S. subjects using the V2 region of the 16S rRNA gene.
  • Fig. S4. Taxonomic distribution across gut samples sorted by the dominant genera within each population.
  • Fig. S5. Prevalence/abundance curves of representative bacterial OTUs in feces (A), oral (B), and skin (C).
  • Fig. S6. ClonalFrame analysis of Escherichia coli strains based on the sequence of seven housekeeping genes.
  • Fig. S7. PCoA plot of Yanomami, Malawian, and Guahibo fecal samples.
  • Fig. S8. Fecal microbiome of non-Bacteroides OTUs in Yhotgun metagenomes of fecal and oral samples with at least 10,000 mapped reads.
  • anomami, Malawian, Guahibo, and U.S. samples.
  • Fig. S9. Bacterial diversity as a function of age and body site.
  • Fig. S10. Procrustes analyses of shotgun metagenomic and 16S rRNA data for different subsets of samples.
  • Fig. S11. Comparison of PICRUSt versus sequenced s
  • Fig. S12. Bacterial genome assembly.
  • References (72–76)

Download PDF

Other Supplementary Material for this manuscript includes the following:

  • Table S7 (Microsoft Excel format). List of all taxa significantly associated with each population in fecal samples as determined by LEfSe analysis.
  • Table S8 (Microsoft Excel format). List of all taxa significantly associated with each population in oral samples as determined by LEfSe analysis.
  • Table S9 (Microsoft Excel format). List of all taxa significantly associated with each population in skin samples as determined by LEfSe analysis.

Files in this Data Supplement: