Science Advances

Supplementary Materials

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  • Fig. S1. Western blot of ZO-1 levels 24 to 96 hours after siRNA transfection (top panel; NT, nontargeting siRNA; C/O, claudin-5 and occludin siRNAs) and densitometric analysis of relative ZO-1 protein levels normalized to NT siRNA sample at each time point (bottom panel; data are means ± SEM).
  • Fig. S2. FITC–Aβ(1–42) peptide was applied to the apical chamber of a Transwell plate 72 hours after siRNA treatment of Bend.3 cells, and its movement across cell monolayers was monitored by fluorescence spectrophotometry over the course of 2 hours (left panel).
  • Fig. S3. FITC-inulin was applied to the basolateral chamber of a Transwell plate 72 hours after siRNA treatment of Bend.3 cells, and its movement across cell monolayers was monitored by fluorescence spectrophotometry over the course of 2 hours.
  • Fig. S4. Down-regulation of claudin-5, occludin, and LRP1 in primary mouse brain endothelial cells and analysis of FITC–Aβ(1–40) movement.
  • Fig. S5. FITC–Aβ(1–40) (10 nM) was applied to the basolateral chamber of a Transwell plate 72 hours after siRNA treatment of Bend.3 cells, and its movement across cell monolayers was assessed by ELISA over the course of 2 hours (left panel).
  • Fig. S6. Relative purity of mouse brain capillary fractions isolated using a dispase/dextran method.
  • Fig. S7. Occludin expression and BBB permeability in APPP/PS1 mice post suppression of tight junctions.

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