Science Advances

Supplementary Materials

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  • Fig. S1. Validation of second-derivative band intensity at 1127 cm−1 in the FTIR spectra as a marker for relative lactate concentration.
  • Fig. S2. Validation of second-derivative band intensity at 1627 cm−1 in the FTIR spectra as a marker for relative concentration of aggregated β sheet proteins and, therefore, protein oxidation.
  • Fig. S3. Correlation of FTIR maps of lipid distribution with H&E histology.
  • Fig. S4. HCA analysis of FTIR maps distinguishes four distinct regions: the molecular layer, granular layer, inner white matter, and hemorrhaged white matter.
  • Fig. S5. Principal components analysis of the average spectra for tissue layers determined from the HCA.
  • Fig. S6. Synchrotron radiation–based (SR) FTIR mapping of cerebellum tissue to identify Mie scattering and electric field standing wave spectra features.
    Fig. S7. SR x-ray fluorescence elemental maps (2-μm step size) of healthy cerebellum blood vessels, showing location of Fe (white arrow) within the wall of the blood vessel.
  • Fig. S8. PIXE elemental maps of healthy and hemorrhaged cerebellum blood vessels.
  • Fig. S9. Resonance Raman spectra (514-nm excitation) collected from hemorrhaged tissue and dried red blood cells, showing characteristic enhanced intensity of hemoglobin bands.
  • References (73–94)

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