Science Advances

Supplementary Materials

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  • Fig. S1. Heat map of the RNA-Seq analysis of NF-κB target genes (list of 150 genes; http://bioinfo.lifl.fr/NF-KB/) comparing the four molecular subtypes of human GBM (TCGA data, downloaded as level 3 normalization counts; classification from www.cbioportal.org).
  • Fig. S2. qRT-PCR analysis of six representative NF-κB target genes in normal brain tissue, HRas-shp53 tumor (n = 5), and shNF1-shp53 tumor (n = 5) samples.
  • Fig. S3. qRT-PCR analysis of six representative MES markers in normal brain tissue and four tumor samples (HRasV12-shp53).
  • Fig. S4. Flow cytometry analysis of 005 BTICs either left untransduced (control) or transduced with a lenti-κB-mCherry reporter.
  • Fig. S5. Silencing IKK2 in NF5310 BTICs.
  • Fig. S6. Silencing IKK2 in Af/fIKK2R53-Cre-ErT2 cells.
  • Fig. S7. Entry of the NBD peptide into the CNS.
  • Fig. S8. Hematoxylin and eosin staining of a tumor section from (A) a representative mouse from the control group at clinical end point and (B) an NBDwt-treated mouse that was sacrificed when the control mice had reached clinical end point.
  • Fig. S9. Treatment of U87 xenograft mice with the NBD peptide.
  • Fig. S10. Timp1 expression.
  • Fig. S11. Timp1 expression in human GBM.
  • Fig. S12. Inducible knockdown of Timp1.
  • Fig. S13. Bioluminescent imaging of a representative mouse from the U87- miRTimp1(−)Dox and U87-miRTimp1(+)Dox groups at the indicated time points.
  • Fig. S14. qRT-PCR analysis of Timp1 in U87luc-miRTimp1 tumors in the absence or presence of Dox in the chow.
  • Fig. S15. Kaplan-Meier survival plot for human glioma samples with differential Timp1 gene expression (National Cancer Institute, REMBRANDT Web site).
  • Table S1. Expression levels of NF-κB target genes in lentivirus-induced GBM.
  • Table S2. List of primers.
  • Table S3. List of antibodies.

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