Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • Section S1. Archaeological samples, radiocarbon dating, and DNA contamination control
  • Section S2. DNA extraction, library preparation, and mitochondrial capture (ACAD)
  • Section S3. DNA extraction and library preparation (UCSC Human Paleogenomics laboratory and HMS Reich laboratory)
  • Section S4. Mitochondrial DNA capture (HMS Reich laboratory)
  • Section S5. Bayesian phylogenetic analysis of mitochondrial data
  • Section S6. Ecological assessment of population sustainability in Beringia
  • Section S7. Bayesian coalescent simulations and evaluation of demographic scenarios
  • Table S1. Date calibrations for ancient mitogenome sequences.
  • Table S2. List of ancient samples, associated metadata, and sequencing results (separate Excel document).
  • Table S3. Complete list of accession numbers for modern mitogenome sequences.
  • Table S4. Polymerase chain reaction (PCR) primers used for mitochondrial long-range PCR.
  • Table S5. Predictive accuracy of the PCMLR model.
  • Table S6. Prediction of the population structure model that fits the observed data.
  • Table S7. Highest predictive accuracy of the PCMLR model for each population structure model when model C is removed from the analysis.
  • Table S8. Prediction of the population structure model that fits the observed data when model C is removed from the analysis.
  • Fig. S1. Location of archaeological sites (see table S2 for detailed information about sites and samples).
  • Fig. S2. Phylogenetic tree of newly sequenced ancient mitogenomes (haplogroup A).
  • Fig. S3. Phylogenetic tree of newly sequenced ancient mitogenomes (haplogroup B).
  • Fig. S4. Phylogenetic tree of newly sequenced ancient mitogenomes (haplogroup C).
  • Fig. S5. Phylogenetic tree of newly sequenced ancient mitogenomes (haplogroup D).
  • Fig. S6. Maximum parsimony phylogenetic trees of the 93 ancient mitogenomes and three replicate data sets of the 87 modern Native American mitogenomes (see table S3).
  • Fig. S7. Extended Bayesian skyline plots of female effective population size for the three replicate data sets.
  • Fig. S8. Bayesian estimates of node ages for haplogroups A2, B2, C1, D1, and D4h3a.
  • Fig. S9. Results of the date-randomization test for temporal sampling structure.
  • Fig. S10. PCA plot of summary statistics for the 15,000 simulations for each of the seven models simulated with BayeSSC (see Fig. 4).
  • Fig. S11. PCA plot of summary statistics for the 15,000 simulations for each of the models simulated with BayeSSC, when model C is removed from the PCA.
  • References (48–86)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2. List of ancient samples, associated metadata, and sequencing results (separate Excel document

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