Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Attachment and growth of hPSCs cultured under various medium conditions.
  • fig. S2. Exogenous adenosine induced osteogenic differentiation of hESCs.
  • fig. S3. Exogenous adenosine induced expressions of osteoblastic markers for hiPSCs.
  • fig. S4. Adenosine-induced osteogenic differentiation of hESCs uses A2bR.
  • fig. S5. In vitro hard tissue–forming ability of hiPSC-derived cells.
  • fig. S6. Minimal hard tissue formation for Ad-hiPSC–laden matrices before implantation.
  • fig. S7. hiPSC-derived osteoblasts (Ad-hiPSCs) facilitate the repair of criticalsized bone defects.
  • fig. S8. Donor hiPSC–derived cells (Ad-hiPSCs) contribute to the regeneration of vascularized neobone tissue.
  • table S1. List of primer sequences used for qPCR analysis.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi format). Minimal calcification from d-hiPSCs in vitro.
  • movie S2 (.avi format). Dense and homogeneous calcified tissue formation from Ad-hiPSCs in vitro.

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