Science Advances

Supplementary Materials

This PDF file includes:

  • table S1. Data collection and refinement statics.
  • table S2. Thermodynamic parameters of CckA-ligand interactions as measured by ITC.
  • table S3. Kinetic parameters of HK/phosphatase CckA_ΔTM.
  • fig. S1. CckA crystal packings.
  • fig. S2. Detailed views of domain interface of CckA_DHp-CA and comparison with c-di-GMP–bound CckA_CA.
  • fig. S3. Sequence alignment between CckA orthologs and paralogs.
  • fig. S4. ITC ligand-binding profiles for several CckA.
  • fig. S5. Structure prediction for PAS2 domain of CckA.
  • fig. S6. Proposed model of noncovalent c-di-GMP–mediated cross-linking of the CA with the DHp domain of CckA.
  • fig. S7. Consensus tree analysis for CckA orthologs and paralogs.
  • fig. S8. Residue conservation in CckA orthologs and paralogs.
  • References (4551)

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Other Supplementary Material for this manuscript includes the following:

  • database S1 (Microsoft Excel format). Bacterial strains used in this study.
  • database S2 (Microsoft Excel format). Plasmids used in this study.
  • database S3 (Microsoft Excel format). Oligonucleotides used in this study.

Files in this Data Supplement: