Science Advances

Supplementary Materials

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  • Supplementary Text
  • table S1. Chromatography program and MRM parameters for GC-MS/MS characterization of DFRC product mix.
  • table S2. Experimental results from the analysis of extract-free whole-cell-wall and enzyme lignin samples of selected eudicots.
  • table S3. Experimental results from the analysis of extract-free whole-cell-wall samples of gymnosperms, magnoliids, and noncommelinid (early) monocots.
  • table S4. Experimental results from the analysis of extract-free whole-cell-wall samples of commelinid monocots.
  • table S5. Experimental results from the analysis of extract-free whole-cell-wall samples of eudicots.
  • table S6. Experimental results from the analysis of extract-free whole-cell-wall samples of plants generated in the enzyme expression study.
  • table S7. Primers used in this study.
  • fig. S1. The monolignol biosynthetic pathway indicating the formation of ML-FAs.
  • fig. S2. The phylogenetic reconstruction of BAHD acyl-CoA ATs is consistent with the convergent evolution of the two feruloyl-CoA monolignol transferases, OsAT5/FMT and AsFMT.
  • fig. S3. Genomic position and gene expression data for the AT5-D1 rice activation-tagged line.
  • fig. S4. OsAt5 expression data and DFRC-released ML-DHpCA conjugates from OsAT5 rice lines.
  • fig. S5. The cell wall compositional differences in OsAT5-D1 straw are predominantly due to the (50 mM TFA, 100°C) insoluble fraction.

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