Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Results
  • fig. S1. Multiple sequence alignment of PadC homologs generated with
    Jalview (64).
  • fig. S2. Spectroscopic and kinetic characterization of TsPadC and MaPadC.
  • fig. S3. Characterization of the IsPadC PSMcc variant.
  • fig. S4. IsPadC crystal and spectral characteristics of dark-state
    crystallized IsPadC.
  • fig. S5. Effect of substrate binding on the overall architecture of IsPadC.
  • fig. S6. Summary of HDX experiments.
  • fig. S7. Individual deuterium incorporation plots of all evaluated peptides.
  • fig. S8. Spectroscopic and kinetic characterization of IsPadC deletion variants.
  • fig. S9. Time course of tryptic digests of IsPadC, MaPadC, and TsPadC under dark and light conditions.
  • fig. S10. Details of SAXS measurements.
  • table S1. Data collection, phasing, and refinement statistics.
  • table S2. Overview of oligonucleotides and buffers.
  • Legends for movies S1 to S4
  • References (62–66)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1(.mp4 format). Changes in conformational dynamics upon red light illumination of IsPadC.
  • movie S2 (.mp4 format). Changes in conformational dynamics upon red light illumination of the IsPadC PSMcc variant.
  • movie S3 (.mp4 format). The influence of effector deletion on conformational dynamics of the dark-state IsPadC PSMcc assembly.
  • movie S4 (.mp4 format). The influence of effector deletion on conformational dynamics of the light-state IsPadC PSMcc assembly.

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