Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Absolute bacterial abundance per bee gut, as measured by 16S rRNA gene copies.
  • fig. S2. Gut community similarities, compared using PCoA of binary Sørensen-Dice dissimilarities at OTU97 clustering.
  • fig. S3. NMDS plots of gut community Sørensen-Dice dissimilarities at OTU97 clustering.
  • fig. S4. UPGMA clustering of communities based on Sørensen-Dice and unweighted UniFrac distances.
  • fig. S5. Linear mixed models testing of the effects of sampling location and host identity on microbiome composition.
  • fig. S6. Ancestral microbiome reconstructions using parsimony for the major corbiculate bacterial phylotypes.
  • fig. S7. Bayesian phylogeny of Lactobacillus Firm-5 based on the tuf gene.
  • fig. S8. Maximum likelihood phylogeny of Lactobacillus Firm-5 based on the tuf gene.
  • fig. S9. Phylogeny of Lactobacillus Firm-5 based on rpoA, and geographic associations of strains.
  • fig. S10. Tanglegram of host bee and Lactobacillus Firm-5 phylogenetic topologies.
  • fig. S11. Gut microbial diversity, as measured by Shannon's H on OTUs clustered at 99.5% similarity.
  • fig. S12. Relationships between bee body size, gut community size (16S copies), and microbiome diversity.
  • fig. S13. Linear models testing of the effects of bee body size, gut community size (16S copies), host phylogenetic affiliation (tribe), and bee colony size on microbiome diversity.
  • table S1. Bee size and colony size estimation and literature sources.
  • table S2. Snodgrassella co-inoculation trials count data.
  • Legends for data files S1 to S5
  • References (122–148)

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Other Supplementary Material for this manuscript includes the following:

  • data file S1. List of samples and associated metadata used in this study.
  • data file S2. OTU97 tables and curated taxonomic assignments.
  • data file S3. OTU99.5 tables summarized by bacterial taxa, from samples rarefied to 5000 reads.
  • data file S4. List of sequences deposited in GenBank.
  • data file S5. Representative sequences from OTU97 and OTU99.5 clustering.

Files in this Data Supplement: