Science Advances

Supplementary Materials

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  • fig. S1. TFIIIC is not associated with CTCF and cohesin in mammalian cells, whereas the association of condensin II and TFIIIC complexes is stable.
  • fig. S2. Knockdown of NCAPH2 did not affect the binding of TFIIIC-220 to the genome.
  • fig. S3. CTS are preferentially localized at the promoter/TSS of highly expressed gene.
  • fig. S4. CTS are correlated with the promoters of active genes.
  • fig. S5. Binding of architectural proteins, active promoter, and enhancer markers at CTS but not at CFTS.
  • fig. S6. CTS have higher CpG islands and GC content than CFTS.
  • fig. S7. Down-regulation of highly transcribed genes upon condensin II knockdown in mESC and HEK293.
  • fig. S8. Histone peptide assays and IP experiments.
  • fig. S9. Effect of reduced H3K4me3 on the binding of NCAPH2 and TFIIIC-220 to CTS.
  • fig. S10. Characterization of CTS at TAD boundaries in mESCs.
  • fig. S11. Characterization of CTS at TAD boundaries in HEK293 cells.
  • fig. S12. CTS are not correlated with LAD or LOCK domains in mESCs.
  • fig. S13. Clustered CTS are associated with stronger TAD boundaries in mESCs.
  • fig. S14. Interactions between two histone gene loci on chr13.
  • fig. S15. Chromatin interactions between two highly expressed gene loci were reduced upon NCAPH2 knockdown.
  • fig. S16. Formation of histone clusters may be disrupted in NCAPH2 knockdown mESCs.
  • table S1. Public ChIP-seq data sets analyzed.

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