Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Differences between dogs and wolves for three behavioral indices used to predict the WBS phenotype.
  • fig. S2. Scree plot of principal components of human-directed social behavior.
  • fig. S3. Scan for positive selection using a bivariate percentile score (XP-EHH and FST) to identify outliers (dashed line; bivariate score, >2) indicated as sites in the 97.5th percentile.
  • fig. S4. Gel electrophoresis banding patterns for three hypersociability-associated SV genotypes.
  • fig. S5. A dot plot to represent the total number of insertions per population of species for each outlier locus.
  • fig. S6. Plots from the ANOVA of the total number of SV insertions at four outlier loci.
  • fig. S7. PCA from 25,510 unlinked genome-wide SNPs from the Affymetrix K9HDSNP array for six wolves and five dogs.
  • fig. S8. SV discovery pipeline.
  • fig. S9. Overlap in number of SVs identified by SVMerge, SoftSearch, and inGAP-sv.
  • table S1. Raw behavioral data.
  • table S2. Data for indices of human-directed social behavior.
  • table S3. Loadings of first three PCs of human-directed social behavior.
  • table S4. Genes in target region on canine chromosome 6.
  • table S5. Sample information and the total number of raw reads compared to the number of processed reads after using cutadapt to trim/clip paired-end sequences.
  • table S6. Outlier clusters on chromosome 6 (CanFam3.1) showing signals of positive selection from XP-EHH.
  • table S7. Summary of de novo annotated structural variants on canine chromosome 6.
  • table S8. De novo annotated structural variants on canine chromosome 6 (coordinates based on CanFam3.1 assembly).
  • table S9. Structural variant summary statistics per individual.
  • table S10. Genic loci associated with indices of human-directed social behavior across dogs and wolves after inclusion of species as a covariate.
  • table S11. Association to species membership.
  • table S12. Predicted functional consequences of SVs.
  • table S13. The significantly enriched (adjusted P < 0.05) gene ontology term from a quantitative association test with each behavioral trait and 544,000 genome-wide SNPs.
  • table S14. The significantly enriched (adjusted P < 0.05) gene ontology term from the univariate regression analysis conducted in GEMMA with each behavioral trait and 544,000 genome-wide SNPs.
  • table S15. Behavioral data and description relative to WBS.
  • table S16. Parameters for in silico annotation of structural variants for the three methods SVMerge, SoftSearch, and InGAP-SV.
  • table S17. Structural variant genotype per individual.
  • table S18. Primer sequences used for PCR and gel-based validation of structural variants.
  • Reference (99)

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Other Supplementary Material for this manuscript includes the following:

  • data file S1 (Microsoft Excel format). Genotypes of the four outlier insertions in wild and domestic canids.

Files in this Data Supplement: