Science Advances

Supplementary Materials

This PDF file includes:

  • Legends for videos S1 to S7
  • fig. S1. MSS analysis results for DC-SIGN clusters.
  • fig. S2. KI (300 mM) does not quench Calcium Orange AM inside the cells.
  • fig. S3. Effect of nocodazole on MT status in MX DC-SIGN cells.
  • fig. S4. Effect of latrunculin A on actin filament status.
  • fig. S5. Conceptualization of a ventral MT occupying a channel within the actin cortex with a putative MT motor driving the directed motion of DC-SIGN.
  • table S1. Maximum instantaneous and average (including stall time) speeds of selected DENV trajectories in the MDDC projection shown in Fig. 5D and video S5.

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Other Supplementary Material for this manuscript includes the following:

  • video S1 (.avi format). Fluorescence video of DC-SIGN clusters exhibiting long, highly directed excursions.
  • video S2 (.avi format). Fluorescence video of EMBD-labeled MTs (green, left) in an MX DC-SIGN cell (red, middle) with superimposed trajectories of DC-SIGN clusters exhibiting highly directed, superdiffusive motion (right).
  • video S3 (.avi format). Quenching of DC-SIGN fluorescence by KI in a TIRF video of MX DC-SIGN cells (to accompany Fig. 3C).
  • video S4 (.avi format). Effect of ciliobrevin on lysosmal transport.
  • video S5 (.avi format). Effect of ciliobrevin on lysosmal transport.
  • video S6 (.avi format). DC-SIGN–directed transport in MX DC-SIGN dendritic projections favors the retrograde direction (to accompany Fig. 5).
  • video S7 (.avi format). When DENV binds to projections on MDDCs, it also undergoes rapid, directed transport.

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