Science Advances

Supplementary Materials

This PDF file includes:

  • Supplementary Materials and Methods
  • fig. S1. Transport of EEs and SVs.
  • fig. S2. Pulse-chase analysis of mEosFPthermo-ChsB near the hyphal tip in the presence of microtubule or actin-depolymerizing drugs.
  • fig. S3. Quantitative analysis of mEosFPthermo-ChsB numbers in the hyphal tip and expression levels.
  • fig. S4. Pulse-chase analysis upon mEosFPthermo-ChsB photoconversion at the hyphal tip.
  • Legends for movies S1 to S15
  • table S1. A. nidulans strains used in this study.
  • Reference (51)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.avi file). Superresolution movie of mEosFPthermo-ChsB clusters generated with the moving-window binning technique (500 frames binning with 50 frames shift).
  • movie S2 (.avi file). Dynamics of ChsB clusters identified by cluster analysis.
  • movie S3 (.avi file). Pulse-chase imaging of mEosFPthermo-ChsB in the wild-type hyphal tip region with local photoconversion ~5 μm behind the hyphal tip.
  • movie S4 (.avi file). Transport of EEs.
  • movie S5 (.avi file). Pulse-chase imaging of mEosFPthermo-TeaR in the wild-type hyphal tip region with a local photoconversion ~5 μm behind the hyphal tip.
  • movie S6 (.mov file). Partial comigration of GFP-ChsB and mCherry-RabA.
  • movie S7 (.avi file). Pulse-chase imaging of mEosFPthermo-ChsB near the hyphal tip in the presence of microtubule drugs (benomyl, 2 μg/ml).
  • movie S8 (.avi file). Pulse-chase imaging of mEosFPthermo-ChsB near the hyphal tip in the presence of actin-depolymerizing drugs (cytochalasin A, 2 μg/ml).
  • movie S9 (.avi file). Pulse-chase imaging of mEosFPthermo-ChsB in the hyphal tip region of the cell from the kinA deletion strains.
  • movie S10 (.avi file). Pulse-chase imaging of mEosFPthermo-ChsB in the hyphal tip region of the cell from the uncA deletion strains.
  • movie S11 (.avi file). Pulse-chase imaging of mEosFPthermo-ChsB in the hyphal tip region of the cell from the myoV deletion strains.
  • movie S12 (.avi file). Pulse-chase imaging after mEosFPthermo-ChsB photoconversion at the hyphal tip of a wild-type cell.
  • movie S13 (.avi file). Pulse-chase imaging after mEosFPthermo-ChsB photoconversion at the hyphal tip in the presence of microtubule drugs (benomyl, 2 μg/ml).
  • movie S14 (.avi file). Pulse-chase imaging after mEosFPthermo-ChsB photoconversion at the hyphal tip in the presence of actin-depolymerizing drugs (cytochalasin A, 2 μg/ml).
  • movie S15 (.avi file). Pulse-chase imaging after mEosFPthermo-ChsB photoconversion at the hyphal tip of a cell from the uncA deletion strain.

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