Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Genome editing of DMD top 12 exons by CRISPR/Cas9.
  • fig. S2. Correction of a large deletion mutation (Del. Ex47 to Ex50) in DMD iPSCs and iPSC-derived cardiomyocytes.
  • fig. S3. Correction of a pseudo-exon mutation (pEx47A) in DMD iPSCs and iPSC-derived cardiomyocytes.
  • fig. S4. Correction of a large duplication mutation (Dup. Ex55 to Ex59) in DMD iPSCs and iPSC-derived cardiomyocytes.
  • fig S5. Dystrophin protein expression rescued by myoediting.
  • fig S6. Cardiomyocyte content and structure in myoedited EHM.
  • table S1. Sequence of primers for top 12 exons.
  • table S2. Sequence of primers for DMD iPSCs.
  • table S3. DNA sequence of corrected iPSC single clones.

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.mp4 format). WT iPSC–derived 3D-EHM on flexible holders.
  • movie S2 (.mp4 format). Uncorrected Del iPSC–derived 3D-EHM on flexible holders.
  • movie S3 (.mp4 format). Del-Cor-SC iPSC–derived 3D-EHM on flexible holders.
  • movie S4 (.mp4 format). Uncorrected pEx iPSC–derived 3D-EHM on flexible holders.
  • movie S5 (.mp4 format). pEx-Cor. iPSC–derived 3D-EHM on flexible holders.
  • movie S6 (.mp4 format). pEx-Cor-SC iPSC–derived 3D-EHM on flexible holders.
  • movie S7 (.mp4 format). Uncorrected Dup iPSC–derived 3D-EHM on flexible holders.
  • movie S8 (.mp4 format). Dup-Cor-SC iPSC–derived 3D-EHM on flexible holders.

Files in this Data Supplement: