Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. PCR analysis of T. muris samples with 16S rRNA gene primers.
  • fig. S2. FISH using a Cy3-labeled probe (NON338) complementary to EUB338 on sections of T. muris adults to control for nonspecific binding.
  • fig. S3. Shannon diversity of all bacteria and the three main phyla detected in the murine microbiota before and after infection and the T. muris microbiota.
  • fig. S4. Community abundance differences were compared at all taxonomic levels to identify significant shifts between groups.
  • fig. S5. Increase in β diversity as a result of infection in the host cacal microbiota, not seen in T. muris.
  • fig. S6. NMDS analysis of host intestinal microbiotas from different mouse strains infected with a high or low dose of T. muris compared to uninfected controls.
  • fig. S7. NMDS analysis of DGGE comparing the microbiota of T. muris isolated from C57BL/6 mice infected with a low dose of T. muris at day 0, day 41, or both days (a single and repeat infection).
  • fig. S8. NMDS analysis of DGGE comparing the microbiota of GF mice that have been reconstituted with a cecal slurry from chronically infected C57BL/6 mice.
  • fig. S9. DGGE of fecal samples from GF mice inoculated with naïve mouse cecal slurry (lanes 1 to 3), pure culture of Bt strain VPI-5482 (lane 4), and GF mice inoculated with Bt (lanes 5 to 8) 12 days after inoculation and those inoculated with Bt at day 35 p.i. (lanes 9 to 13).
  • fig. S10. Parasite-specific IgG2a/c antibody in serum from low dose–infected GF mice that had been reconstituted with Bt strain VPI-5482, with a naïve FS from a WT C57BL/6 mouse and WT C57BL/6 control mice.
  • Legends for tables S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • table S1 (Microsoft Excel format). Species shared between groups: naïve mice, infected mice, and T. muris microbiotas.
  • table S2 (Microsoft Excel format). P values and FDR-adjusted P values for differences in bacterial proportions at different taxonomic levels between groups.

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