Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Distribution of measured eggshell nanostructures by AFM.
  • fig. S2. Eggshell nanostructure area measurements.
  • fig. S3. Internal nanocrystal misalignments in the PL of chicken eggshell.
  • fig. S4. TEM showing eggshell nanostructure.
  • fig. S5. Semiquantification of OPN immunostaining across the eggshell thickness.
  • fig. S6. Effect of OPN on nanostructure size in synthetic calcite crystals.
  • fig. S7. OPN induces nanostructure in synthetic calcite crystals.
  • fig. S8. Absence of nanostructure in synthetic control calcite crystal (no added OPN).
  • fig. S9. Electron microscopy of a FIB section showing nanostructure in a synthetic calcite crystal grown with OPN (5.9 μM).
  • fig. S10. Nanoindentation displacement curves for eggshell and synthetic calcite crystals grown in OPN (5.9 μM).

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.mov format). 3D reconstruction from a tilt series of the upper PL of avian chicken eggshell G. gallus.
  • movie S2 (.mov format). 3D reconstruction of nanodomains found in the upper PL of the eggshell.
  • movie S3 (.mov format). 3D reconstruction from a tilt series of the synthetic calcite crystal grown with 5.9 μM OPN.
  • movie S4 (.mov format). 3D reconstruction of a nanostructured region found in the synthetic calcite crystal grown with 5.9 μM OPN.

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