Science Advances

Supplementary Materials

This PDF file includes:

  • note S1. Attenuation-compensation of an Airy beam light sheet
  • note S2. Attenuation-compensation of a Bessel beam light sheet
  • note S3. Modification of deconvolution protocol incorporating attenuation and attenuation-compensation
  • note S4. Effect of incorrect attenuation estimation on deconvolution
  • note S5. Determination of specimen attenuation
  • note S6. Sample-based geometric effects on attenuation
  • note S7. Attenuation-compensation of multiphoton excitation Airy and Bessel light sheets
  • fig. S1. Pupil functions of attenuation-compensated Airy and Bessel beams.
  • fig. S2. Look-up tables of achievable attenuation-compensation for Airy and Bessel beam parameters.
  • fig. S3. Line profiles through simulated images of a 1D resolution target shown in Fig. 1 (main text).
  • fig. S4. Simulated images: Effect on deconvolution from error in estimation of attenuation.
  • fig. S5. Sample induced geometric effects on attenuation profile across the FOV.
  • fig. S6. Attenuation-compensated LSM in a scattering sample.
  • fig. S7. Local SBR and CNR measured for data shown in Fig. 4 (main text).
  • fig. S8. Local SBR and CNR measured for data shown in Fig. 5 (main text).
  • fig. S9. Light-sheet intensity profiles for the data shown in Fig. 5 (main text).
  • fig. S10. Effect of attenuation and attenuation-compensation on two-photon excitation SPIM and DLSM Airy light sheet.
  • fig. S11. Effect of attenuation and attenuation-compensation on two-photon excitation Bessel beam.
  • fig. S12. Effect of attenuation and attenuation-compensation on two-photon excitation DSLM Bessel light sheet.
  • fig. S13. Schematic of attenuation-compensated Airy light-sheet microscope.
  • table S1. Experimental parameters for all data shown in main text.
  • table S2. FOV of two-photon excitation SPIM and DLSM Airy light sheet with attenuation and attenuation-compensation.
  • table S3. FOV of two-photon excitation Bessel beam and DLSM Bessel light sheet with attenuation and attenuation-compensation.

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