Science Advances

Supplementary Materials

This PDF file includes:

  • fig. S1. Comparison of experimental HS-AFM image of an FlhAC monomer with a simulated AFM image of the crystal structure of FlhAC.
  • fig. S2. Effect of phospholipids on FlhAC ring formation.
  • fig. S3. Effect on motility and flagellar formation of alanine substitutions of residues involved in interactions of FlhAL with its neighboring subunit in the FlhAC crystal.
  • fig. S4. Effect of phospholipids on ring formation of FlhAC mutant variants.
  • fig. S5. Effect of FlhA mutations on interactions of FlhAC with FlgN and FliJ.
  • fig. S6. Effect of depletion of FliH and FliI on the export function of FlhA(L401A).
  • fig. S7. Effect of the FlhA(F459A) mutation on FlhAC ring formation.
  • table S1. Salmonella strains and plasmids used in this study.
  • Legends for movies S1 to S5
  • References (40–45)

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Other Supplementary Material for this manuscript includes the following:

  • movie S1 (.mov format). Real-time imaging of FlhAC ring assembly on mica.
  • movie S2 (.mov format). Typical HS-AFM imaging of wild-type FlhAC placed on mica surface.
  • movie S3 (.mov format). Typical HS-AFM imaging of FlhAC38K placed on mica surface.
  • movie S4 (.mov format). Typical HS-AFM imaging of wild-type FlhAC placed on mica-supported planar phospholipid bilayers.
  • movie S5 (.mov format). Typical HS-AFM imaging of FlhAC38K placed on mica-supported planar phospholipid bilayers.

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