Science Advances

Supplementary Materials

This PDF file includes:

  • Fig. S1. Computational approach to identify parallel amino acid substitutions between pairs of independent lineages.
  • Fig. S2. Phylogeny of the 30 placental mammals.
  • Fig. S3. Radical parallel substitutions in conserved positions between microbat and dolphin in hearing-related genes.
  • Fig. S4. Convergence of microbat and dolphin Myh2 toward Myh4.
  • Fig. S5. Myh4 is deleted in the cetacean lineage.
  • Fig. S6. Unassembled genomic and RNA reads confirm the absence of Myh4 in the cetacean lineage.
  • Fig. S7. The amount of aligning intronic sequence distinguishes Myh orthologs from paralogs.
  • Table S1. Classification of all parallel amino acid substitutions detected by reconstructing ancestral sequences with a maximum likelihood and a Bayesian approach.
  • Table S2. Functional enrichments of proteins with parallel substitutions between the microbat (little brown bat) and the bottlenose dolphin.
  • Table S3. Pairs of independent branches and their number of parallel substitutions in fast-twitch versus slow-twitch fiber proteins.
  • Table S4. GC-biased gene conversion alone can potentially explain only one of the seven parallel substitutions in the fast-twitch muscle proteins.
  • Table S5. Top 30 genes with a significantly higher expression level in the anterior cricothyroid muscle compared to breast muscle of P. parnellii.
  • Table S6. Expression of calsequestrin, Ca2+ ATPase, myosin heavy chain, and myosin light chain genes in the anterior cricothyroid muscle and the breast muscle of the echolocating P. parnellii bat.
  • References (4955)

Download PDF

Files in this Data Supplement: