Table 1 Comparison among three methodologies.
Fabricated deviceExoQuickUltracentrifugation
Collected objectsExosomes
Microvesicles
EV-free miRNAs
Exosomes
Microvesicles
Exosomes
Collection mechanismElectrostatic interaction
between nanowire surface
charge and collected objects
Polymer-based capture of objects ranging
from 60 to 180 nm in diameter, according to
the kit manufacturer’s instruction manual
Balance between applied
forces and density of the
collected objects
Sample volume1 ml1 ml1 ml for small RNA quantification
20 ml for urinary miRNA profiling
Processing time40 min870 min300 min
Collection efficiency (small RNA yield)0.194 ± 0.028 ng/μl0.120 ± 0.015 ng/μl0.159 ± 0.077 ng/μl
Extraction species of urinary
miRNAs being identified
749, 822, 1111 (n = 3)337, 355, 491 (n = 3)171, 261, 352 (n = 3)
200 to 300*

*From Cheng et al. (31).