Table 1 Quality metrics for this assembly compared to previous donkey genome assemblies.

The number of annotated genes (lower than that in previous assemblies) shows a better homologous correspondence with the horse gene set (see Gene annotation).

This studyHuang et al. (15)Orlando et al. (2)
N50 contigs140.3 kb66.7 kb6.38 kb
N50 scaffolds15.4 Mb3.8 Mb100.94 kb
Coverage61.2×42.4×12.4×
Total bases2.320 Gb2.391 Gb2.293 Gb
Largest scaffold84.20 Mb17.06 Mb1.09 Mb
Unresolved bases per 100 kb1121.611384.934128.43
Total number of predicted protein-coding genes18,98423,850*24,156

*Calculated using one isoform per gene and 42,247 total transcripts.