Superresolution microscopy reveals a dynamic picture of cell polarity maintenance during directional growth

Yuji Ishitsuka; Natasha Savage; Yiming Li; Anna Bergs; Nathalie Grün; Daria Kohler; Rebecca Donnelly; G. Ulrich Nienhaus; Reinhard Fischer; Norio Takeshita

doi:10.1126/sciadv.1500947

Research ArticleCELL BIOLOGY

Superresolution microscopy reveals a dynamic picture of cell polarity maintenance during directional growth

Yuji Ishitsuka¹,
Natasha Savage²,
Yiming Li¹,
Anna Bergs³,
Nathalie Grün³,
Daria Kohler¹,
Rebecca Donnelly²,
G. Ulrich Nienhaus¹,⁴,⁵,⁶,*,
Reinhard Fischer³,* and
Norio Takeshita³,⁷,*

¹Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany.
²Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK.
³Department of Microbiology, Institute for Applied Biosciences, KIT, 76187 Karlsruhe, Germany.
⁴Institute of Nanotechnology, KIT, 76344 Eggenstein-Leopoldshafen, Germany.
⁵Institute of Toxicology and Genetics, KIT, 76344 Eggenstein-Leopoldshafen, Germany.
⁶Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
⁷Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.

↵*Corresponding author. E-mail: uli{at}uiuc.edu (G.U.N.); reinhard.fischer{at}kit.edu (R.F.); norio.takeshita{at}kit.edu (N.T.)

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Science Advances 13 Nov 2015:
Vol. 1, no. 10, e1500947
DOI: 10.1126/sciadv.1500947

Yuji Ishitsuka

Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany.

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Natasha Savage

Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK.

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Yiming Li

Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany.

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Anna Bergs

Department of Microbiology, Institute for Applied Biosciences, KIT, 76187 Karlsruhe, Germany.

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Nathalie Grün

Department of Microbiology, Institute for Applied Biosciences, KIT, 76187 Karlsruhe, Germany.

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Daria Kohler

Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany.

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Rebecca Donnelly

Department of Functional and Comparative Genomics, Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB, UK.

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G. Ulrich Nienhaus

Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), 76131 Karlsruhe, Germany.Institute of Nanotechnology, KIT, 76344 Eggenstein-Leopoldshafen, Germany.Institute of Toxicology and Genetics, KIT, 76344 Eggenstein-Leopoldshafen, Germany.Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

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For correspondence: uli@uiuc.edu reinhard.fischer@kit.edu norio.takeshita@kit.edu

Reinhard Fischer

Department of Microbiology, Institute for Applied Biosciences, KIT, 76187 Karlsruhe, Germany.

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For correspondence: uli@uiuc.edu reinhard.fischer@kit.edu norio.takeshita@kit.edu

Norio Takeshita

Department of Microbiology, Institute for Applied Biosciences, KIT, 76187 Karlsruhe, Germany.Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.

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ORCID record for Norio Takeshita
For correspondence: uli@uiuc.edu reinhard.fischer@kit.edu norio.takeshita@kit.edu

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Superresolution microscopy reveals a dynamic picture of cell polarity maintenance during directional growth

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