Research ArticleNEUROSCIENCE

Therapy using implanted organic bioelectronics

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Science Advances  08 May 2015:
Vol. 1, no. 4, e1500039
DOI: 10.1126/sciadv.1500039

Figures

  • Fig. 1 The implantable OEIP and its spinal target.

    (A) Photograph of the device. (B) Schematic illustration: electrical connection (left); reservoir with internal electrode (center); delivery channel and outlets (right). Total length 120 mm; reservoir diameter 6 mm, length 60 mm; delivery tip width 1.2 mm, length 40 mm, thickness about 0.2 mm. (C) Depiction of the four outlets aligned with the sites where the sciatic nerve bundles enter the spinal cord [reused with permission from (24)]. The figure shows a human spinal cord, but the roots L3-L6 refer to the root levels of a rat spinal cord.

  • Fig. 2 Geometry of the implantable device.

    (Left) The pattern of the delivery channel leading from the reservoir out to the four delivery points (ends of the “fingers”). Dimensions are given in micrometers. (Right) A simplified equivalent circuit. RL > RD > RGRE, where RE represents the ionic resistance of the electrolyte and counter electrode.

  • Fig. 3 Demonstration of simultaneous delivery using pH.

    (A and B) Microscope images of the delivery tip (A) before and (B) during delivery of H+. The dashed lines indicate the edge of the encapsulation, defining the delivery points. The scale bar refers to both (A) and (B). The clouds of lower pH can be seen as the red regions at the delivery tips. (C) Approximate pH profiles at the four delivery tips. The change in pH shows the highly localized regions of lower pH.

  • Fig. 4 Therapeutic effect of GABA delivery in vivo.

    (A) WT as a function of time for OEIP delivery of GABA at two delivery rates and H+ control delivery. The asterisks indicate significance of multiple comparisons between all three traces. P = 0.0024, 0.0014, 0.0286, 0.0002, 0.0113, and 0.0002 for 45, 60, 75, 90, 105, and 120 min, respectively. (B) WT as a function of delivered therapeutic. P = 0.0303, 0.0079, 0.019, 0.0179, 0.0079, and 0.0286 for 3.6, 4.5, 5.4, 6.3, 7.2, and 9.1 mC, respectively. Data in both (A) and (B) are represented as means ± SEM.

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