StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity

Sophia L. Samodelov; Hannes M. Beyer; Xiujie Guo; Maximilian Augustin; Kun-Peng Jia; Lina Baz; Oliver Ebenhöh; Peter Beyer; Wilfried Weber; Salim Al-Babili; Matias D. Zurbriggen

doi:10.1126/sciadv.1601266

Research ArticleLIFE SCIENCES

StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity

Sophia L. Samodelov¹,²,
Hannes M. Beyer²,³,
Xiujie Guo⁴,
Maximilian Augustin³,*,
Kun-Peng Jia⁴,
Lina Baz⁴,
Oliver Ebenhöh⁵,
Peter Beyer³,
Wilfried Weber²,³,⁶,
Salim Al-Babili⁴,†,‡ and
Matias D. Zurbriggen¹,†,‡

¹Institute of Synthetic Biology and Cluster of Excellence on Plant Sciences (CEPLAS), University of Düsseldorf, Düsseldorf, Germany.
²Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany.
³Faculty of Biology, University of Freiburg, Freiburg, Germany.
⁴King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division, Center for Desert Agriculture, 23955-6900 Thuwal, Saudi Arabia.
⁵Institute of Quantitative and Theoretical Biology and CEPLAS, University of Düsseldorf, Düsseldorf, Germany.
⁶BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.

↵‡Corresponding author. Email: matias.zurbriggen{at}uni-duesseldorf.de (M.D.Z.); salim.babili{at}kaust.edu.sa (S.A.-B.)

↵* Present address: Roche Diagnostics GmbH, Sandhofer Strasse 116, Mannheim, Germany.
↵† These authors contributed equally to this work.

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Science Advances 04 Nov 2016:
Vol. 2, no. 11, e1601266
DOI: 10.1126/sciadv.1601266

Sophia L. Samodelov

Institute of Synthetic Biology and Cluster of Excellence on Plant Sciences (CEPLAS), University of Düsseldorf, Düsseldorf, Germany.Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany.

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Hannes M. Beyer

Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany.Faculty of Biology, University of Freiburg, Freiburg, Germany.

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ORCID record for Hannes M. Beyer

Xiujie Guo

King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division, Center for Desert Agriculture, 23955-6900 Thuwal, Saudi Arabia.

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Maximilian Augustin

Faculty of Biology, University of Freiburg, Freiburg, Germany.

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Kun-Peng Jia

King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division, Center for Desert Agriculture, 23955-6900 Thuwal, Saudi Arabia.

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Lina Baz

King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division, Center for Desert Agriculture, 23955-6900 Thuwal, Saudi Arabia.

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Oliver Ebenhöh

Institute of Quantitative and Theoretical Biology and CEPLAS, University of Düsseldorf, Düsseldorf, Germany.

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ORCID record for Oliver Ebenhöh

Peter Beyer

Faculty of Biology, University of Freiburg, Freiburg, Germany.

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Wilfried Weber

Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany.Faculty of Biology, University of Freiburg, Freiburg, Germany.BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.

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Salim Al-Babili

King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division, Center for Desert Agriculture, 23955-6900 Thuwal, Saudi Arabia.

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For correspondence: matias.zurbriggen@uni-duesseldorf.de salim.babili@kaust.edu.sa

Matias D. Zurbriggen

Institute of Synthetic Biology and Cluster of Excellence on Plant Sciences (CEPLAS), University of Düsseldorf, Düsseldorf, Germany.

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For correspondence: matias.zurbriggen@uni-duesseldorf.de salim.babili@kaust.edu.sa

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StrigoQuant: A genetically encoded biosensor for quantifying strigolactone activity and specificity

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