Research ArticleHIGH RESOLUTION IMAGING

Development of ultrasound bioprobe for biological imaging

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Science Advances  25 Oct 2017:
Vol. 3, no. 10, e1701176
DOI: 10.1126/sciadv.1701176
  • Fig. 1 Schematic illustration of an ultrasound bioprobe.

    Customized piezo-transducers underneath the sample and the cantilever provide the flexural vibrations. The AFM mechanical probe detects the subsurface mechanical contrast.

  • Fig. 2 Subsurface imaging of magnetic particles embeded in silica core shell.

    (A) Schematic illustration of the magnetic core nanostructure embedded in the refractory silica core shell–based molecular marker. (B) AFM topographical image showing well dispersed silica core shell nanostructures. (C) Normal tapping-mode phase image that doesn’t show any phase contrast coming from embedded particles in silica core. (D) Phase contrast from subsurface magnetic nanoparticles enclosed in core shell nanoparticles at nanoscale spatial resolution. The magnetic core, silica core, and receptor layer are identified in the ultrasonic phase. fs and fc are the sample and cantilever ultrasonic frequencies, respectively.

  • Fig. 3 Intracellular fibers imaging of endothelial cells stimulated with thrombin.

    (A) AFM topographical image of the ECs altered by the addition of thrombin. (B) Ultrasound bioprobe phase image showing notable contrast from intracellular fibers. Intracellular fibers are predominantly seen in the ultrasound phase image along with stretched gaps and subcellular phase contrast in the nuclear region of the cells.

Supplementary Materials

  • Supplementary material for this article is available at http://advances.sciencemag.org/cgi/content/full/3/10/e1701176/DC1

    fig. S1. High-resolution TEM image of the magnetic particles embedded in the silica core shell.

    fig. S2. Ultrasound bioprobe image of the ECs when treated with thrombin.

    fig. S3. Detection of difference (beat) frequency when the feedback control electronics of probe is on and off.

  • Supplementary Materials

    This PDF file includes:

    • fig. S1. High-resolution TEM image of the magnetic particles embedded in the silica core shell.
    • fig. S2. Ultrasound bioprobe image of the ECs when treated with thrombin.
    • fig. S3. Detection of difference (beat) frequency when the feedback control electronics of probe is on and off.

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