Research ArticleBIOCHEMISTRY
Fast iodide-SAD phasing for high-throughput membrane protein structure determination
- Igor Melnikov1,*,
- Vitaly Polovinkin2,3,4,*,
- View ORCID ProfileKirill Kovalev3,4,
- View ORCID ProfileIvan Gushchin3,4,
- Mikhail Shevtsov4,
- Vitaly Shevchenko3,4,5,
- Alexey Mishin4,
- Alexey Alekseev3,4,
- View ORCID ProfileFrancisco Rodriguez-Valera6,
- Valentin Borshchevskiy4,
- Vadim Cherezov4,7,
- Gordon A. Leonard1,
- Valentin Gordeliy2,3,4,† and
- Alexander Popov1,†
- 1European Synchrotron Radiation Facility, 38043 Grenoble, France.
- 2Institut de Biologie Structurale Jean-Pierre Ebel, Université Grenoble Alpes–Commissariat à l'Energie Atomique et aux Energies Alternatives–CNRS, F-38000 Grenoble, France.
- 3Institute of Complex Systems (ICS), ICS-6, Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.
- 4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
- 5Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany.
- 6Department of Vegetal Production and Microbiology, Universidad Miguel Hernández de Elche, San Juan de Alicante, Valencia, Spain.
- 7Departments of Chemistry, Biological Sciences, and Physics & Astronomy, Bridge Institute, University of Southern California, Los Angeles, CA 90089, USA.
- ↵†Corresponding author. Email: valentin.gordeliy{at}ibs.fr (V.G.); apopov{at}esrf.fr (A.P.)
↵* These authors contributed equally to this work.
See allHide authors and affiliations
Science Advances 12 May 2017:
Vol. 3, no. 5, e1602952
DOI: 10.1126/sciadv.1602952
Vol. 3, no. 5, e1602952
DOI: 10.1126/sciadv.1602952
Igor Melnikov
1European Synchrotron Radiation Facility, 38043 Grenoble, France.
Vitaly Polovinkin
2Institut de Biologie Structurale Jean-Pierre Ebel, Université Grenoble Alpes–Commissariat à l'Energie Atomique et aux Energies Alternatives–CNRS, F-38000 Grenoble, France.
3Institute of Complex Systems (ICS), ICS-6, Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
Kirill Kovalev
3Institute of Complex Systems (ICS), ICS-6, Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
Ivan Gushchin
3Institute of Complex Systems (ICS), ICS-6, Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
Mikhail Shevtsov
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
Vitaly Shevchenko
3Institute of Complex Systems (ICS), ICS-6, Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
5Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany.
Alexey Mishin
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
Alexey Alekseev
3Institute of Complex Systems (ICS), ICS-6, Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
Francisco Rodriguez-Valera
6Department of Vegetal Production and Microbiology, Universidad Miguel Hernández de Elche, San Juan de Alicante, Valencia, Spain.
Valentin Borshchevskiy
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
Vadim Cherezov
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
7Departments of Chemistry, Biological Sciences, and Physics & Astronomy, Bridge Institute, University of Southern California, Los Angeles, CA 90089, USA.
Gordon A. Leonard
1European Synchrotron Radiation Facility, 38043 Grenoble, France.
Valentin Gordeliy
2Institut de Biologie Structurale Jean-Pierre Ebel, Université Grenoble Alpes–Commissariat à l'Energie Atomique et aux Energies Alternatives–CNRS, F-38000 Grenoble, France.
3Institute of Complex Systems (ICS), ICS-6, Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany.
4Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Moscow Region, Russia.
Alexander Popov
1European Synchrotron Radiation Facility, 38043 Grenoble, France.
