A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9

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Science Advances  04 Aug 2017:
Vol. 3, no. 8, eaao0027
DOI: 10.1126/sciadv.aao0027

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  • RE: Alternatives to Crispr-Cas based Gene editing
    • Aitzaz Bin Sultan Rai, Clinical Research Fellow, King Edward Medical University, Lahore, Pakistan
    • Other Contributors:
      • Rizwan Naseer, Resident, King edward Medical University
      • Waqar Ilahi, Research Fellow, University of Pennsylvania Hospital system
      • Noorikiran Haris, Consultant, King Edward Medical University
      • Hassan Bin Attique, Fellow, University Of Connecticut
      • Arooma Ismail, Resident, King Edward Medical University

    We read the article with great interest which is an interesting piece of research in ever the growing field of gene modification. However, Any technique based on CRISPR-cas system is inherently bound with some limitations.

    There are newer technologies on the horizon which offer more robust and plausible ways for gene modification. The details of such techniques are beyond this brief e-letter, However We would like to mention Argonautes (NgAgo)(1)editing enzyme Cpf1,(2) Enzyme system C2c2(3), Nanoparticle based solutions are just few of several examples where we can do more beyond ZAPPING the DNA.

    Despite these newer and emerging technologies which have considerable potential we would still be reliant on CRISPR Cas system for quite sometime. But the take home message is we must not limit ourselves on one promising technique and expand our horizons to more avenues of research specially in multi-disciplinary settings.

    1) Gao, F., Shen, X.Z., Jiang, F., Wu, Y. and Han, C., 2016. DNA-guided genome editing using the Natronobacterium gregoryi Argonaute. Nature biotechnology, 34(7), pp.768-773.

    2)Yamano, T., Nishimasu, H., Zetsche, B., Hirano, H., Slaymaker, I.M., Li, Y., Fedorova, I., Nakane, T., Makarova, K.S., Koonin, E.V. and Ishitani, R., 2016. Crystal structure of Cpf1 in complex with guide RNA and target DNA. Cell, 165(4), pp.949-962.

    3) East-Seletsky, A., O’Connell, M.R., Knight, S.C., Burstein, D., Cate, J.H., Tjian, R. and Doudna, J.A.,...

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    Competing Interests: None declared.

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