Research ArticleCANCER
Mapping a functional cancer genome atlas of tumor suppressors in mouse liver using AAV-CRISPR–mediated direct in vivo screening
- Guangchuan Wang1,2,*,
- Ryan D. Chow1,2,3,*,
- Lupeng Ye1,2,
- Christopher D. Guzman1,2,4,5,
- Xiaoyun Dai1,2,
- Matthew B. Dong1,2,3,5,
- Feng Zhang6,7,
- Phillip A. Sharp8,9,
- Randall J. Platt10,11,† and
- Sidi Chen1,2,4,12,13,14,†
- 1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
- 2Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
- 3MD-PhD Program, Yale University School of Medicine, New Haven, CT 06510, USA.
- 4Immunobiology Program, Yale University School of Medicine, New Haven, CT 06510, USA.
- 5Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
- 6Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard University, 75 Ames Street, Cambridge, MA 02142, USA.
- 7Department of Biological Engineering, MIT, Cambridge, MA 02142, USA.
- 8Koch Institute for Integrative Cancer Research, MIT, 77 Massachusetts Avenue, Cambridge, MA 02139–4307, USA.
- 9Department of Biology, MIT, Cambridge, MA 02139–4307, USA.
- 10Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, 4058 Basel, Switzerland.
- 11Department of Chemistry, University of Basel, Petersplatz 1, 4003 Basel, Switzerland.
- 12Biological and Biomedical Sciences Program, Yale University School of Medicine, West Haven, CT 06516, USA.
- 13Comprehensive Cancer Center, Yale University School of Medicine, West Haven, CT 06516, USA.
- 14Stem Cell Center, Yale University School of Medicine, West Haven, CT 06516, USA.
- ↵†Corresponding author. Email: sidi.chen{at}yale.edu (S.C.); rplatt{at}ethz.ch (R.J.P.)
↵* These authors contributed equally to this work.
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Science Advances 28 Feb 2018:
Vol. 4, no. 2, eaao5508
DOI: 10.1126/sciadv.aao5508
Vol. 4, no. 2, eaao5508
DOI: 10.1126/sciadv.aao5508
Guangchuan Wang
1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
2Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
Ryan D. Chow
1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
2Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
3MD-PhD Program, Yale University School of Medicine, New Haven, CT 06510, USA.
Lupeng Ye
1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
2Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
Christopher D. Guzman
1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
2Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
4Immunobiology Program, Yale University School of Medicine, New Haven, CT 06510, USA.
5Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Xiaoyun Dai
1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
2Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
Matthew B. Dong
1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
2Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
3MD-PhD Program, Yale University School of Medicine, New Haven, CT 06510, USA.
5Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Feng Zhang
6Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard University, 75 Ames Street, Cambridge, MA 02142, USA.
7Department of Biological Engineering, MIT, Cambridge, MA 02142, USA.
Phillip A. Sharp
8Koch Institute for Integrative Cancer Research, MIT, 77 Massachusetts Avenue, Cambridge, MA 02139–4307, USA.
9Department of Biology, MIT, Cambridge, MA 02139–4307, USA.
Randall J. Platt
10Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, 4058 Basel, Switzerland.
11Department of Chemistry, University of Basel, Petersplatz 1, 4003 Basel, Switzerland.
Sidi Chen
1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
2Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
4Immunobiology Program, Yale University School of Medicine, New Haven, CT 06510, USA.
12Biological and Biomedical Sciences Program, Yale University School of Medicine, West Haven, CT 06516, USA.
13Comprehensive Cancer Center, Yale University School of Medicine, West Haven, CT 06516, USA.
14Stem Cell Center, Yale University School of Medicine, West Haven, CT 06516, USA.
