Research ArticleCANCER
Mapping a functional cancer genome atlas of tumor suppressors in mouse liver using AAV-CRISPR–mediated direct in vivo screening
- Guangchuan Wang1,2,*,
- Ryan D. Chow1,2,3,*,
- Lupeng Ye1,2,
- Christopher D. Guzman1,2,4,5,
- Xiaoyun Dai1,2,
- Matthew B. Dong1,2,3,5,
- Feng Zhang6,7,
- Phillip A. Sharp8,9,
- Randall J. Platt10,11,† and
- Sidi Chen1,2,4,12,13,14,†
- 1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.
- 2Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
- 3MD-PhD Program, Yale University School of Medicine, New Haven, CT 06510, USA.
- 4Immunobiology Program, Yale University School of Medicine, New Haven, CT 06510, USA.
- 5Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
- 6Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard University, 75 Ames Street, Cambridge, MA 02142, USA.
- 7Department of Biological Engineering, MIT, Cambridge, MA 02142, USA.
- 8Koch Institute for Integrative Cancer Research, MIT, 77 Massachusetts Avenue, Cambridge, MA 02139–4307, USA.
- 9Department of Biology, MIT, Cambridge, MA 02139–4307, USA.
- 10Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, 4058 Basel, Switzerland.
- 11Department of Chemistry, University of Basel, Petersplatz 1, 4003 Basel, Switzerland.
- 12Biological and Biomedical Sciences Program, Yale University School of Medicine, West Haven, CT 06516, USA.
- 13Comprehensive Cancer Center, Yale University School of Medicine, West Haven, CT 06516, USA.
- 14Stem Cell Center, Yale University School of Medicine, West Haven, CT 06516, USA.
- ↵†Corresponding author. Email: sidi.chen{at}yale.edu (S.C.); rplatt{at}ethz.ch (R.J.P.)
↵* These authors contributed equally to this work.
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Science Advances 28 Feb 2018:
Vol. 4, no. 2, eaao5508
DOI: 10.1126/sciadv.aao5508
Vol. 4, no. 2, eaao5508
DOI: 10.1126/sciadv.aao5508
Guangchuan Wang
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
Ryan D. Chow
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.MD-PhD Program, Yale University School of Medicine, New Haven, CT 06510, USA.
Lupeng Ye
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
Christopher D. Guzman
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.Immunobiology Program, Yale University School of Medicine, New Haven, CT 06510, USA.Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Xiaoyun Dai
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.
Matthew B. Dong
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.MD-PhD Program, Yale University School of Medicine, New Haven, CT 06510, USA.Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Feng Zhang
Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard University, 75 Ames Street, Cambridge, MA 02142, USA.Department of Biological Engineering, MIT, Cambridge, MA 02142, USA.
Phillip A. Sharp
Koch Institute for Integrative Cancer Research, MIT, 77 Massachusetts Avenue, Cambridge, MA 02139–4307, USA.Department of Biology, MIT, Cambridge, MA 02139–4307, USA.
Randall J. Platt
Department of Biosystems Science and Engineering, ETH Zürich, Mattenstrasse 26, 4058 Basel, Switzerland.Department of Chemistry, University of Basel, Petersplatz 1, 4003 Basel, Switzerland.
Sidi Chen
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.Systems Biology Institute, Yale University School of Medicine, 850 West Campus Drive, West Haven, CT 06516, USA.Immunobiology Program, Yale University School of Medicine, New Haven, CT 06510, USA.Biological and Biomedical Sciences Program, Yale University School of Medicine, West Haven, CT 06516, USA.Comprehensive Cancer Center, Yale University School of Medicine, West Haven, CT 06516, USA.Stem Cell Center, Yale University School of Medicine, West Haven, CT 06516, USA.
