Antiviral activity of a purine synthesis enzyme reveals a key role of deamidation in regulating protein nuclear import

Junhua Li; Jun Zhao; Simin Xu; Shu Zhang; Junjie Zhang; Jun Xiao; Ruoyun Gao; Mao Tian; Yi Zeng; Katie Lee; Vera Tarakanova; Ke Lan; Hao Feng; Pinghui Feng

doi:10.1126/sciadv.aaw7373

Research ArticleBIOCHEMISTRY

Antiviral activity of a purine synthesis enzyme reveals a key role of deamidation in regulating protein nuclear import

Junhua Li¹,*,
Jun Zhao¹,
Simin Xu¹,
Shu Zhang¹,
Junjie Zhang¹,
Jun Xiao¹,²,
Ruoyun Gao¹,
Mao Tian¹,
Yi Zeng¹,³,
Katie Lee¹,
Vera Tarakanova⁴,
Ke Lan⁵,
Hao Feng² and
Pinghui Feng ¹,⁵,†

¹Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.
²Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, P.R. China.
³Department of Pathology and Immunology, Youjiang Medical University for Nationalities, Baise, Guangxi 533000, P.R. China.
⁴Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
⁵State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, P.R. China.

↵†Corresponding author. Email: pinghui.feng{at}usc.edu

↵* Present address: State Key Laboratory of Agricultural Microbiology, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, P.R. China.

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Science Advances 09 Oct 2019:
Vol. 5, no. 10, eaaw7373
DOI: 10.1126/sciadv.aaw7373

Junhua Li

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.

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Jun Zhao

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.

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Simin Xu

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.

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Shu Zhang

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.

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Junjie Zhang

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.

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Jun Xiao

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, P.R. China.

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Ruoyun Gao

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.

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Mao Tian

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.

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Yi Zeng

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.Department of Pathology and Immunology, Youjiang Medical University for Nationalities, Baise, Guangxi 533000, P.R. China.

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Katie Lee

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.

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Vera Tarakanova

Department of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.

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Ke Lan

State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, P.R. China.

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Hao Feng

Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, P.R. China.

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Pinghui Feng

Section of Infection and Immunity, Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, 925 W. 34th Street, Los Angeles, CA 90089-0641, USA.State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, P.R. China.

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For correspondence: pinghui.feng@usc.edu

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Antiviral activity of a purine synthesis enzyme reveals a key role of deamidation in regulating protein nuclear import

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