Research ArticleBIOCHEMISTRY

Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method

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Science Advances  09 Oct 2019:
Vol. 5, no. 10, eaaw8451
DOI: 10.1126/sciadv.aaw8451

Article Information

vol. 5 no. 10

Online ISSN: 
  • Received for publication March 29, 2019
  • Accepted for publication September 17, 2019
  • .

Author Information

  1. Yumeng Tan1,*,
  2. Yong Zhang1,*,
  3. Yunbin Han1,2,
  4. Hao Liu1,
  5. Haifeng Chen1,
  6. Fuqiang Ma1,3,
  7. Stephen G. Withers4,
  8. Yan Feng1 and
  9. Guangyu Yang1,
  1. 1State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  2. 2State Key Laboratory Cultivation Base for TCM Quality and Efficacy, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
  3. 3CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, China.
  4. 4Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T 1Z1, Canada.
  1. Corresponding author. Email: yanggy{at}
  • * These authors contributed equally to this work.


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Article usage: October 2019 to January 2020

Oct 201951335331016
Nov 201944847195
Dec 201925657115
Jan 2020932444

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