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The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation

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Science Advances  13 Nov 2019:
Vol. 5, no. 11, eaaw9807
DOI: 10.1126/sciadv.aaw9807

Article Information

vol. 5 no. 11

Online ISSN: 
History: 
  • Received for publication April 23, 2019
  • Accepted for publication September 17, 2019
  • .

Author Information

  1. Qian Zhang1,2,3,*,
  2. Fengcai Wen1,2,3,*,
  3. Siqi Zhang1,2,3,
  4. Jiachuan Jin4,
  5. Lulu Bi1,
  6. Ying Lu3,5,
  7. Ming Li3,5,
  8. Xu-Guang Xi6,
  9. Xingxu Huang1,
  10. Bin Shen4, and
  11. Bo Sun1,
  1. 1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  2. 2Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
  3. 3University of Chinese Academy of Sciences, Beijing 100049, China.
  4. 4State Key Laboratory of Reproductive Medicine, Center for Global Health, Nanjing Medical University, Nanjing 211166, China.
  5. 5Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
  6. 6LBPA, IDA, ENS de Cachan, CNRS, Université Paris-Saclay, Cachan F-94235, France.
  1. Corresponding author. Email: sunbo{at}shanghaitech.edu.cn (B.Su); binshen{at}njmu.edu.cn (B.Sh)
  • * These authors contributed equally to this work.

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