Research ArticleLIFE SCIENCES
The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
- View ORCID ProfileQian Zhang1,2,3,*,
- Fengcai Wen1,2,3,*,
- Siqi Zhang1,2,3,
- Jiachuan Jin4,
- View ORCID ProfileLulu Bi1,
- View ORCID ProfileYing Lu3,5,
- View ORCID ProfileMing Li3,5,
- View ORCID ProfileXu-Guang Xi6,
- Xingxu Huang1,
- View ORCID ProfileBin Shen4,† and
- View ORCID ProfileBo Sun1,†
- 1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
- 2Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
- 3University of Chinese Academy of Sciences, Beijing 100049, China.
- 4State Key Laboratory of Reproductive Medicine, Center for Global Health, Nanjing Medical University, Nanjing 211166, China.
- 5Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
- 6LBPA, IDA, ENS de Cachan, CNRS, Université Paris-Saclay, Cachan F-94235, France.
- ↵†Corresponding author. Email: sunbo{at}shanghaitech.edu.cn (B.Su); binshen{at}njmu.edu.cn (B.Sh)
↵* These authors contributed equally to this work.
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Science Advances 13 Nov 2019:
Vol. 5, no. 11, eaaw9807
DOI: 10.1126/sciadv.aaw9807
Vol. 5, no. 11, eaaw9807
DOI: 10.1126/sciadv.aaw9807
Qian Zhang
1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
2Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
3University of Chinese Academy of Sciences, Beijing 100049, China.
Fengcai Wen
1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
2Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
3University of Chinese Academy of Sciences, Beijing 100049, China.
Siqi Zhang
1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
2Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
3University of Chinese Academy of Sciences, Beijing 100049, China.
Jiachuan Jin
4State Key Laboratory of Reproductive Medicine, Center for Global Health, Nanjing Medical University, Nanjing 211166, China.
Lulu Bi
1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Ying Lu
3University of Chinese Academy of Sciences, Beijing 100049, China.
5Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
Ming Li
3University of Chinese Academy of Sciences, Beijing 100049, China.
5Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
Xu-Guang Xi
6LBPA, IDA, ENS de Cachan, CNRS, Université Paris-Saclay, Cachan F-94235, France.
Xingxu Huang
1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Bin Shen
4State Key Laboratory of Reproductive Medicine, Center for Global Health, Nanjing Medical University, Nanjing 211166, China.
Bo Sun
1School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
