Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution

Grant E. Duclos; Vitor H. Teixeira; Patrick Autissier; Yaron B. Gesthalter; Marjan A. Reinders-Luinge; Robert Terrano; Yves M. Dumas; Gang Liu; Sarah A. Mazzilli; Corry-Anke Brandsma; Maarten van den Berge; Sam M. Janes; Wim Timens; Marc E. Lenburg; Avrum Spira; Joshua D. Campbell; Jennifer Beane

doi:10.1126/sciadv.aaw3413

Research ArticleCELL BIOLOGY

Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution

Grant E. Duclos ¹,*,
Vitor H. Teixeira²,
Patrick Autissier³,
Yaron B. Gesthalter⁴,
Marjan A. Reinders-Luinge⁵,
Robert Terrano¹,
Yves M. Dumas¹,
Gang Liu¹,
Sarah A. Mazzilli¹,
Corry-Anke Brandsma⁵,
Maarten van den Berge⁶,
Sam M. Janes²,⁷,
Wim Timens⁵,
Marc E. Lenburg¹,
Avrum Spira¹,⁸,
Joshua D. Campbell ¹,*,† and
Jennifer Beane ¹,*,†

¹Department of Medicine, Boston University School of Medicine, Boston, MA, USA.
²Lungs for Living Research Centre, UCL Respiratory, University College London, London, UK.
³Boston University Flow Cytometry Core Facility, Boston University School of Medicine, Boston, MA, USA.
⁴Department of Medicine, University of California San Francisco School of Medicine, San Francisco, CA, USA.
⁵University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Groningen, Netherlands.
⁶University of Groningen, University Medical Center Groningen, Department of Pulmonary Diseases, Groningen, Netherlands.
⁷Department of Thoracic Medicine, University College London Hospital, London, UK.
⁸Johnson & Johnson Innovation, Cambridge, MA, USA.

↵*Corresponding author. Email: duclosgr{at}bu.edu (G.E.D.); camp{at}bu.edu (J.D.C.); jbeane{at}bu.edu (J.B.)

↵† These authors contributed equally to this work.

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Science Advances 11 Dec 2019:
Vol. 5, no. 12, eaaw3413
DOI: 10.1126/sciadv.aaw3413

Grant E. Duclos

1Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

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For correspondence: duclosgr@bu.edu camp@bu.edu jbeane@bu.edu

Vitor H. Teixeira

2Lungs for Living Research Centre, UCL Respiratory, University College London, London, UK.

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Patrick Autissier

3Boston University Flow Cytometry Core Facility, Boston University School of Medicine, Boston, MA, USA.

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Yaron B. Gesthalter

4Department of Medicine, University of California San Francisco School of Medicine, San Francisco, CA, USA.

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Marjan A. Reinders-Luinge

5University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Groningen, Netherlands.

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Robert Terrano

1Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

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Yves M. Dumas

1Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

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Gang Liu

1Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

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Sarah A. Mazzilli

1Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

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Corry-Anke Brandsma

5University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Groningen, Netherlands.

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Maarten van den Berge

6University of Groningen, University Medical Center Groningen, Department of Pulmonary Diseases, Groningen, Netherlands.

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Sam M. Janes

2Lungs for Living Research Centre, UCL Respiratory, University College London, London, UK.

7Department of Thoracic Medicine, University College London Hospital, London, UK.

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Wim Timens

5University of Groningen, University Medical Center Groningen, Department of Pathology and Medical Biology, Groningen, Netherlands.

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Marc E. Lenburg

1Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

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Avrum Spira

1Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

8Johnson & Johnson Innovation, Cambridge, MA, USA.

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Joshua D. Campbell

1Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

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For correspondence: duclosgr@bu.edu camp@bu.edu jbeane@bu.edu

Jennifer Beane

1Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

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For correspondence: duclosgr@bu.edu camp@bu.edu jbeane@bu.edu

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Characterizing smoking-induced transcriptional heterogeneity in the human bronchial epithelium at single-cell resolution

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