Research ArticleBIOPHYSICS

A hidden state in the turnover of a functioning membrane protein complex

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Science Advances  20 Mar 2019:
Vol. 5, no. 3, eaau6885
DOI: 10.1126/sciadv.aau6885

Supplementary Materials

  • Supplementary material for this article is available at http://advances.sciencemag.org/cgi/content/full/5/3/eaau6885/DC1

    Section S1. The dwell time distribution for the two-state model

    Section S2. The dwell time distribution for the three-state model

    Fig. S1. The motor speed at high load as a function of the number of stators.

    Fig. S2. The distributions of stator number for motors at steady states at various induction levels.

    Fig. S3. The dwell time distribution at stator numbers from 1 to 11 for JY21 induced with 0, 2, and 5 μM IPTG (from left to right).

    Fig. S4. The dwell time distributions for two types of intervals.

    Fig. S5. The dwell time distributions for the two types of “On” intervals.

    Fig. S6. The dwell time distribution at stator numbers from 1 to 11 for the wild-type strain JY27.

    Fig. S7. Data from the MotB plug-deletion strain (SM1 carrying pBAD33MotBΔplug) with induction of 0.001% arabinose.

    Fig. S8. Other possible three-state models.

    Fig. S9. An example of simulated speed trace analyzed by the step-finding algorithm.

    Fig. S10. The interval distributions from the simulated traces using different values of the parameters vm and tm in the step-finding algorithm.

  • Supplementary Materials

    This PDF file includes:

    • Section S1. The dwell time distribution for the two-state model
    • Section S2. The dwell time distribution for the three-state model
    • Fig. S1. The motor speed at high load as a function of the number of stators.
    • Fig. S2. The distributions of stator number for motors at steady states at various induction levels.
    • Fig. S3. The dwell time distribution at stator numbers from 1 to 11 for JY21 induced with 0, 2, and 5 μM IPTG (from left to right).
    • Fig. S4. The dwell time distributions for two types of intervals.
    • Fig. S5. The dwell time distributions for the two types of “On” intervals.
    • Fig. S6. The dwell time distribution at stator numbers from 1 to 11 for the wild-type strain JY27.
    • Fig. S7. Data from the MotB plug-deletion strain (SM1 carrying pBAD33MotBΔplug) with induction of 0.001% arabinose.
    • Fig. S8. Other possible three-state models.
    • Fig. S9. An example of simulated speed trace analyzed by the step-finding algorithm.
    • Fig. S10. The interval distributions from the simulated traces using different values of the parameters vm and tm in the step-finding algorithm.

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