Research ArticleAPPLIED SCIENCES AND ENGINEERING
Electrospray sample injection for single-particle imaging with x-ray lasers
- Johan Bielecki1,2,*,
- Max F. Hantke1,3,*,†,
- Benedikt J. Daurer1,4,*,
- Hemanth K. N. Reddy1,
- Dirk Hasse1,
- Daniel S. D. Larsson1,
- Laura H. Gunn1,
- Martin Svenda1,5,
- Anna Munke1,
- Jonas A. Sellberg1,5,
- Leonie Flueckiger6,
- Alberto Pietrini1,
- Carl Nettelblad1,7,
- Ida Lundholm1,
- Gunilla Carlsson1,
- Kenta Okamoto1,
- Nicusor Timneanu1,8,
- Daniel Westphal1,
- Olena Kulyk9,
- Akifumi Higashiura10,11,
- Gijs van der Schot1,12,
- Ne-Te Duane Loh4,13,
- Taylor E. Wysong14,
- Christoph Bostedt15,16,
- Tais Gorkhover14,
- Bianca Iwan17,18,
- M. Marvin Seibert1,
- Timur Osipov14,
- Peter Walter14,
- Philip Hart14,
- Maximilian Bucher15,
- Anatoli Ulmer19,
- Dipanwita Ray14,
- Gabriella Carini14,‡,
- Ken R. Ferguson14,
- Inger Andersson1,
- Jakob Andreasson9,20,
- Janos Hajdu1,9 and
- Filipe R. N. C. Maia1,21,§
- 1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
- 2European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany.
- 3Chemistry Research Laboratory, Department of Chemistry, Oxford University, 12 Mansfield Rd, Oxford OX1 3TA, UK.
- 4Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117557, Singapore.
- 5Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden.
- 6ARC Centre of Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe University, Melbourne, Victoria 3086, Australia.
- 7Division of Scientific Computing, Department of Information Technology, Science for Life Laboratory, Uppsala University, Lägerhyddsvägen 2 (Box 337), SE-751 05 Uppsala, Sweden.
- 8Department of Physics and Astronomy, Uppsala University, Box 516, SE-75120 Uppsala, Sweden.
- 9Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.
- 10Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan
- 11Department of Virology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan
- 12Cryo-Electron Microscopy, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, Netherlands
- 13Department of Physics, National University of Singapore, Singapore, Singapore.
- 14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
- 15Chemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, IL 60439, USA.
- 16Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208, USA.
- 17Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
- 18Institut für Quantenoptik, Leibniz Universität Hannover, Welfengarten 1, 30167 Hannover, Germany.
- 19Institut für Optik und Atomare Physik, Technische Universität Berlin, 10623 Berlin, Germany.
- 20Condensed Matter Physics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden.
- 21NERSC, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
- ↵§Corresponding author. Email: filipe{at}xray.bmc.uu.se
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Science Advances 03 May 2019:
Vol. 5, no. 5, eaav8801
DOI: 10.1126/sciadv.aav8801
Vol. 5, no. 5, eaav8801
DOI: 10.1126/sciadv.aav8801
Johan Bielecki
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany.
Max F. Hantke
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.Chemistry Research Laboratory, Department of Chemistry, Oxford University, 12 Mansfield Rd, Oxford OX1 3TA, UK.
Benedikt J. Daurer
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117557, Singapore.
Hemanth K. N. Reddy
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Dirk Hasse
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Daniel S. D. Larsson
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Laura H. Gunn
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Martin Svenda
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden.
Anna Munke
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Jonas A. Sellberg
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden.
Leonie Flueckiger
ARC Centre of Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe University, Melbourne, Victoria 3086, Australia.
Alberto Pietrini
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Carl Nettelblad
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.Division of Scientific Computing, Department of Information Technology, Science for Life Laboratory, Uppsala University, Lägerhyddsvägen 2 (Box 337), SE-751 05 Uppsala, Sweden.
Ida Lundholm
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Gunilla Carlsson
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Kenta Okamoto
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Nicusor Timneanu
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.Department of Physics and Astronomy, Uppsala University, Box 516, SE-75120 Uppsala, Sweden.
Daniel Westphal
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Olena Kulyk
Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.
Akifumi Higashiura
Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, JapanDepartment of Virology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan
Gijs van der Schot
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.Cryo-Electron Microscopy, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, Netherlands
Ne-Te Duane Loh
Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117557, Singapore.Department of Physics, National University of Singapore, Singapore, Singapore.
Taylor E. Wysong
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Christoph Bostedt
Chemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, IL 60439, USA.Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208, USA.
Tais Gorkhover
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Bianca Iwan
Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.Institut für Quantenoptik, Leibniz Universität Hannover, Welfengarten 1, 30167 Hannover, Germany.
M. Marvin Seibert
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Timur Osipov
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Peter Walter
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Philip Hart
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Maximilian Bucher
Chemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, IL 60439, USA.
Anatoli Ulmer
Institut für Optik und Atomare Physik, Technische Universität Berlin, 10623 Berlin, Germany.
Dipanwita Ray
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Gabriella Carini
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Ken R. Ferguson
Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Inger Andersson
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Jakob Andreasson
Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.Condensed Matter Physics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden.
Janos Hajdu
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.
Filipe R. N. C. Maia
Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.NERSC, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
