Research ArticleAPPLIED SCIENCES AND ENGINEERING
Electrospray sample injection for single-particle imaging with x-ray lasers
- View ORCID ProfileJohan Bielecki1,2,*,
- View ORCID ProfileMax F. Hantke1,3,*,†,
- View ORCID ProfileBenedikt J. Daurer1,4,*,
- Hemanth K. N. Reddy1,
- View ORCID ProfileDirk Hasse1,
- View ORCID ProfileDaniel S. D. Larsson1,
- View ORCID ProfileLaura H. Gunn1,
- Martin Svenda1,5,
- View ORCID ProfileAnna Munke1,
- View ORCID ProfileJonas A. Sellberg1,5,
- Leonie Flueckiger6,
- View ORCID ProfileAlberto Pietrini1,
- View ORCID ProfileCarl Nettelblad1,7,
- Ida Lundholm1,
- View ORCID ProfileGunilla Carlsson1,
- Kenta Okamoto1,
- View ORCID ProfileNicusor Timneanu1,8,
- Daniel Westphal1,
- Olena Kulyk9,
- View ORCID ProfileAkifumi Higashiura10,11,
- View ORCID ProfileGijs van der Schot1,12,
- View ORCID ProfileNe-Te Duane Loh4,13,
- Taylor E. Wysong14,
- Christoph Bostedt15,16,
- Tais Gorkhover14,
- View ORCID ProfileBianca Iwan17,18,
- M. Marvin Seibert1,
- Timur Osipov14,
- Peter Walter14,
- View ORCID ProfilePhilip Hart14,
- View ORCID ProfileMaximilian Bucher15,
- Anatoli Ulmer19,
- Dipanwita Ray14,
- Gabriella Carini14,‡,
- Ken R. Ferguson14,
- View ORCID ProfileInger Andersson1,
- View ORCID ProfileJakob Andreasson9,20,
- View ORCID ProfileJanos Hajdu1,9 and
- View ORCID ProfileFilipe R. N. C. Maia1,21,§
- 1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
- 2European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany.
- 3Chemistry Research Laboratory, Department of Chemistry, Oxford University, 12 Mansfield Rd, Oxford OX1 3TA, UK.
- 4Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117557, Singapore.
- 5Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden.
- 6ARC Centre of Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe University, Melbourne, Victoria 3086, Australia.
- 7Division of Scientific Computing, Department of Information Technology, Science for Life Laboratory, Uppsala University, Lägerhyddsvägen 2 (Box 337), SE-751 05 Uppsala, Sweden.
- 8Department of Physics and Astronomy, Uppsala University, Box 516, SE-75120 Uppsala, Sweden.
- 9Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.
- 10Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan
- 11Department of Virology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan
- 12Cryo-Electron Microscopy, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, Netherlands
- 13Department of Physics, National University of Singapore, Singapore, Singapore.
- 14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
- 15Chemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, IL 60439, USA.
- 16Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208, USA.
- 17Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
- 18Institut für Quantenoptik, Leibniz Universität Hannover, Welfengarten 1, 30167 Hannover, Germany.
- 19Institut für Optik und Atomare Physik, Technische Universität Berlin, 10623 Berlin, Germany.
- 20Condensed Matter Physics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden.
- 21NERSC, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
- ↵§Corresponding author. Email: filipe{at}xray.bmc.uu.se
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Science Advances 03 May 2019:
Vol. 5, no. 5, eaav8801
DOI: 10.1126/sciadv.aav8801
Vol. 5, no. 5, eaav8801
DOI: 10.1126/sciadv.aav8801
Johan Bielecki
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
2European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany.
Max F. Hantke
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
3Chemistry Research Laboratory, Department of Chemistry, Oxford University, 12 Mansfield Rd, Oxford OX1 3TA, UK.
Benedikt J. Daurer
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
4Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117557, Singapore.
Hemanth K. N. Reddy
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Dirk Hasse
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Daniel S. D. Larsson
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Laura H. Gunn
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Martin Svenda
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
5Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden.
Anna Munke
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Jonas A. Sellberg
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
5Biomedical and X-ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden.
Leonie Flueckiger
6ARC Centre of Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe University, Melbourne, Victoria 3086, Australia.
Alberto Pietrini
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Carl Nettelblad
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
7Division of Scientific Computing, Department of Information Technology, Science for Life Laboratory, Uppsala University, Lägerhyddsvägen 2 (Box 337), SE-751 05 Uppsala, Sweden.
Ida Lundholm
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Gunilla Carlsson
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Kenta Okamoto
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Nicusor Timneanu
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
8Department of Physics and Astronomy, Uppsala University, Box 516, SE-75120 Uppsala, Sweden.
Daniel Westphal
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Olena Kulyk
9Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.
Akifumi Higashiura
10Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan
11Department of Virology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan
Gijs van der Schot
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
12Cryo-Electron Microscopy, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, Netherlands
Ne-Te Duane Loh
4Centre for BioImaging Sciences, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117557, Singapore.
13Department of Physics, National University of Singapore, Singapore, Singapore.
Taylor E. Wysong
14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Christoph Bostedt
15Chemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, IL 60439, USA.
16Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208, USA.
Tais Gorkhover
14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Bianca Iwan
17Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
18Institut für Quantenoptik, Leibniz Universität Hannover, Welfengarten 1, 30167 Hannover, Germany.
M. Marvin Seibert
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Timur Osipov
14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Peter Walter
14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Philip Hart
14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Maximilian Bucher
15Chemical Sciences and Engineering Division, Argonne National Laboratory, Argonne, IL 60439, USA.
Anatoli Ulmer
19Institut für Optik und Atomare Physik, Technische Universität Berlin, 10623 Berlin, Germany.
Dipanwita Ray
14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Gabriella Carini
14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Ken R. Ferguson
14Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Inger Andersson
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
Jakob Andreasson
9Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.
20Condensed Matter Physics, Department of Physics, Chalmers University of Technology, Gothenburg, Sweden.
Janos Hajdu
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
9Institute of Physics, ELI Beamlines, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ-18221 Prague, Czech Republic.
Filipe R. N. C. Maia
1Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
21NERSC, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
