Research ArticleIMMUNOLOGY

Functional diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering

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Science Advances  28 Aug 2019:
Vol. 5, no. 8, eaaw1822
DOI: 10.1126/sciadv.aaw1822

Article Information

vol. 5 no. 8

Online ISSN: 
History: 
  • Received for publication November 26, 2018
  • Accepted for publication July 25, 2019
  • .

Author Information

  1. Johan M. S. van der Schoot1,
  2. Felix L. Fennemann1,
  3. Michael Valente1,
  4. Yusuf Dolen1,
  5. Iris M. Hagemans1,
  6. Anouk M. D. Becker1,
  7. Camille M. Le Gall1,
  8. Duco van Dalen1,
  9. Alper Cevirgel1,
  10. Jaco A. C. van Bruggen2,*,
  11. Melanie Engelfriet2,
  12. Tomislav Caval3,
  13. Arthur E. H. Bentlage4,
  14. Marieke F. Fransen5,
  15. Maaike Nederend6,
  16. Jeanette H. W. Leusen6,
  17. Albert J. R. Heck3,
  18. Gestur Vidarsson4,
  19. Carl G. Figdor1,
  20. Martijn Verdoes1, and
  21. Ferenc A. Scheeren7,
  1. 1Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein 26, 6525 GA Nijmegen, Netherlands.
  2. 2Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, Netherlands.
  3. 3Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, Netherlands.
  4. 4Sanquin Research, Department of Experimental Immunohematology, Amsterdam, The Netherlands, and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Plesmanlaan 125, Amsterdam 1066 CX, Netherlands.
  5. 5Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Albinusdreef 2, 2333 ZA Leiden, Netherlands.
  6. 6Laboratory for Translational Immunology, UMC Utrecht, Utrecht, Netherlands.
  7. 7Department of Medical Oncology, Leiden University Medical Center (LUMC), Albinusdreef 2, 2333 ZA Leiden, Netherlands.
  1. Corresponding author. Email: martijn.verdoes{at}radboudumc.nl (M.Ve.); f.a.scheeren{at}lumc.nl (F.A.S.)
  • * Present address: Department of Experimental Immunology, Amsterdam University Medical Center, Amsterdam, Netherlands.

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