Research ArticleSTRUCTURAL BIOLOGY

Synthetic self-assembling ADDomer platform for highly efficient vaccination by genetically encoded multiepitope display

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Science Advances  25 Sep 2019:
Vol. 5, no. 9, eaaw2853
DOI: 10.1126/sciadv.aaw2853

Abstract

Self-assembling virus-like particles represent highly attractive tools for developing next-generation vaccines and protein therapeutics. We created ADDomer, an adenovirus-derived multimeric protein-based self-assembling nanoparticle scaffold engineered to facilitate plug-and-play display of multiple immunogenic epitopes from pathogens. We used cryo–electron microscopy at near-atomic resolution and implemented novel, cost-effective, high-performance cloud computing to reveal architectural features in unprecedented detail. We analyzed ADDomer interaction with components of the immune system and developed a promising first-in-kind ADDomer-based vaccine candidate to combat emerging Chikungunya infectious disease, exemplifying the potential of our approach.

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