Super-resolution nanoscopy by coherent control on nanoparticle emission

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Science Advances  17 Apr 2020:
Vol. 6, no. 16, eaaw6579
DOI: 10.1126/sciadv.aaw6579
  • Fig. 1 Setup and demonstration of SNAC.

    (A) A pulse shaper is inserted into the femtosecond pump beam of a two-photon fluorescence microscope system. (B) Four emitters under a series of SP (SP1, etc.) display independent response (emission intensity) to SPs’ excitation. (C to F) The fluorescence imaging of gold NRs under different SPs. (G) Corresponding position of the gold NR emitters in the film, shown in the SEM image. (H) Fluorescence imaging of ZnCdS quantum dot film. (I) Periodical response of three emitters marked by red dashed circles in (H) under two SPs. fps, frames per second. (J) Particles’ relative amplitude oscillation (RAO); 20% (±10%) of the RAO is obtained under the SNAC modulation.

  • Fig. 2 Multiple periodical excitation and image processing.

    (A) Four adjacent emitters, S1 to S4, are excited by four SPs periodically. (B) Simulated optical images under four SP excitation in one period. (C) The C42=6combined images and FFT processed six images. (D) Each FFT image is resolved by MG fitting. (E) Summing of images in (D) to generate final output.

  • Fig. 3 Simulation for resolving adjacent emitters and line structure marked with emitters.

    (A) Simulated fluorescent imaging of two adjacent emitters, apart from 30 to 150 nm (pixel = 50 nm, λ_emi = 480 nm). The background includes random Poisson and fixed pattern noise, SNR = 3. (B) Super-resolution image by SNAC-MG. (C) SNAC-MG, SRRF, SOFI, and RL deconvolution algorithms are listed for comparing. The simulation of X-bar cross line shape structure is shown in (D) to (I). (D) Sparse emitters’ position on X-bar. (E) Simulated optical image with pixel = 50 nm, λ_emi = 480 nm, and SNR = 3. (F to I) Super-resolution reconstruction results from SNAC-MG, SRRF, SOFI, and RL deconvolution, respectively.

  • Fig. 4 Super-resolution imaging of a 2D panda.

    (A) A simulated complex 2D structure containing adjacent points and sophisticated line structures. (B) Optical images are simulated with pixel = 50 nm, λ_emi = 480 nm, and SNR = 3. (C) One of the FFT-strengthened CN2 images. (D) Super-resolution reconstruction result of SNAC-MG. (E) Zoomed-in super-resolution image of the panda’s eye, foot, and navel areas in (D) by SNAC-MG, SRRF, SOFI, and RL deconvolution. The positioning accuracy of line structures is marked in SNAC-MG images.

  • Fig. 5 SNAC-MG reconstruction for PMMA-QD films.

    (A to D) Optical images of a PMMA-QD film pumped with four SPs in a period. (E) Reconstructed image based on SNAC-MG. (F) Zoomed-in image of the yellow box in (E). Three blue dashed lines mark two adjacent points for analysis. (G to I) Profile of nos. 1, 2, and 3 dashed lines in (F), respectively.

  • Fig. 6 Data of COS7-QD625–labeled cell of SNAC reconstruction.

    (A) One-photon fluorescence image of a reticular vascular part of COS7 cells labeled by QD625. (B and H) Two-photon excited fluorescence images in wide field for red and blue marked areas in (A). (C to G and I to M) The super-resolution reconstruction results of the marked box parts in (A) of SNAC-MG, MG, SOFI, and RL deconvolution. The Full Width Half Maximum (FWHM) of five lines in the inset of (C) is 31.8, 32.1, 31.1, 44.6, and 39.0 nm, from left to right. The peak distance in the inset of (I) is 95.3 nm.

Supplementary Materials

  • Supplementary Materials

    Super-resolution nanoscopy by coherent control on nanoparticle emission

    Congyue Liu, Wei Liu, Shufeng Wang, Hongjia Li, Zhilong Lv, Fa Zhang, Donghui Zhang, Junlin Teng, Tao Zheng, Donghai Li, Mingshu Zhang, Pingyong Xu, Qihuang Gong

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