Research ArticleOCEANOGRAPHY

Linking extracellular enzymes to phylogeny indicates a predominantly particle-associated lifestyle of deep-sea prokaryotes

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Science Advances  15 Apr 2020:
Vol. 6, no. 16, eaaz4354
DOI: 10.1126/sciadv.aaz4354
  • Fig. 1 Increasing contribution of genes tentatively encoding secretory CAZymes and peptidases with depth.

    Percent distribution (A), Shannon index (B), and Bray-Curtis dissimilarity–based β-diversity (C) for CAZyme and peptidase encoding genes. Box shows median and interquartile range (IQR); whiskers show 1.5 × IQR of the lower and upper quartiles or range; and outliers extend to the data range. Statistics are based on Wilcoxon test. Letters are used to indicate statistical significance (P < 0.05); a shared letter means no significant difference. Epi, epipelagic (n = 216); Meso, mesopelagic (n = 68); Bathy, bathypelagic (n = 54); OMZ (n = 7).

  • Fig. 2 Phylogenetic affiliation and functional classification of genes encoding bacterial CAZymes and peptidases throughout the water column.

    Taxonomic variability at the phylum level (class level for Proteobacteria) of genes encoding CAZymes (A) and peptidases (C); functional composition of genes encoding CAZymes (B) and peptidases (D). Color bars along x axes indicate samples from different depth: green, epipelagic; light blue, mesopelagic; dark blue, bathypelagic; sandy yellow, OMZ.

  • Fig. 3 Phylogenetic affiliation and functional classification of transcripts for gene encoding bacterial CAZymes and peptidases in the bathypelagic ocean.

    Taxonomic variability at the phylum level (class level for Proteobacteria) of transcripts for genes encoding CAZymes (A) and peptidases (C); functional composition of transcripts for genes encoding CAZymes (B) and peptidases (D).

  • Fig. 4 Phylogenetic affiliation and functional classification of bacterial CAZymes and peptidases throughout water column.

    Taxonomic variability at the phylum level (class level for Proteobacteria) of CAZymes (A) and peptidases (C); functional composition of CAZymes (B) and peptidases (D). Color bars along x axes indicate samples from different depth: green, epipelagic; light blue, mesopelagic; dark blue, bathypelagic; sandy yellow, OMZ. Missing data are in white gap.

  • Fig. 5 Percentage of genes encoding secretory CAZymes and peptidases of Alphaproteobacteria and Gammaproteobacteria increasing with depth.

    (A and C), Alphaproteobacteria; (B and D), Gammaproteobacteria. Box shows median and IQR; whiskers show 1.5 × IQR of the lower and upper quartiles or range; outliers extend to the data range. Statistics are based on Wilcoxon test, and letters are used to show statistical significance (P < 0.05); a shared letter means no significant difference. Epipelagic (n = 216); mesopelagic (n = 68); bathypelagic (n = 54); OMZ (n = 7).

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