Research ArticleNEUROSCIENCE

Altered d-glucose in brain parenchyma and cerebrospinal fluid of early Alzheimer’s disease detected by dynamic glucose-enhanced MRI

See allHide authors and affiliations

Science Advances  13 May 2020:
Vol. 6, no. 20, eaba3884
DOI: 10.1126/sciadv.aba3884
  • Fig. 1 Schematic diagram of the glucose transportation in the brain.

    Glucose from the artery enters parenchyma brain and CSF through glucose transporters in the periarterial space, including glucose transporters in blood-CSF barrier (BCSFB) and blood-brain barrier (BBB). A large portion of CSF recirculates to the parenchyma brain and finally drains interstitial fluid (ISF) clearance. μin, uptake rate; μout, clearance rate.

  • Fig. 2 DGE MRI results for brain parenchyma of WT and APP/PS1 mice.

    Dynamic difference images before and after d-glucose infusion for WT (A and B) and APP/PS1 (C and D) mice at 6M (A and C) and 16M (B and D). DGE images were averaged over sets of 15 for display (18 of 270). Experimental (solid line) and fitted (dashed line) parenchymal DGE curves for WT (6M, n = 5) and APP/PS1 (6M, n = 5) mice (E), as well as WT (16M, n = 5) and APP/PS1 (16M, n = 5) mice (F). Comparison of fitted uptake parameters Smax (G) and μin (H) between WT and APP/PS1 mice for two age groups (6M and 16M). Significance levels: *P < 0.05 and ***P < 0.001.

  • Fig. 3 DGE MRI results for CSF of WT and APP/PS1 mice.

    Dynamic difference images before and after d-glucose infusion for WT (A and B) and APP/PS1 (C and D) mice at 6M (A and C) and 16M (B and D). DGE images were averaged over sets of 15 for display (18 of 270). Experimental (solid line) and fitted (dashed line) CSF DGE curves for WT (6M, n = 5) and APP/PS1 (6M, n = 5) mice (E), as well as WT (16M, n = 5) and APP/PS1 (16M, n = 5) mice (F). Comparison of fitted uptake and clearance parameters Smax (G), μin (H), and μout (I) between WT and APP/PS1 mice for two age groups (6M and 16M). Significance levels: *P < 0.05 and ***P < 0.001.

  • Fig. 4 Regional DGE MRI comparison between WT and AD.

    (A) T2 anatomical image with four regions indicated [yellow, cerebral cortex (CX); purple, hippocampus (HC); pink, thalamus (TH); and cyan, entorhinal cortex (EC)]. DGE curves for both age groups and mouse types are compared for CX (B), HC (C), TH (D), and EC (E). Regional Smax (F) and μin (G) comparison between WT (6M and 16M) and APP/PS1 (6M and 16M). Significance levels: *P < 0.05, **P < 0.01, and ***P< 0.001.

  • Table 1 Comparison of Smax and the parenchymal DGE signal difference at 60 min.

    ns, not significant.

    Smax (%)ΔS(60 min) (%)Smax − ΔS(60 min) (%)
    WTAPPPWTAPPPWTAPPP
    6M1.66 ± 0.592.16 ± 0.38ns1.29 ± 0.432.15 ± 0.32**0.37 ± 0.270.01 ± 0.22*
    16M1.62 ± 0.510.87 ± 0.09*1.21 ± 0.400.74 ± 0.08*0.41 ± 0.260.13 ± 0.09*

Supplementary Materials

  • Supplementary Materials

    Altered D-glucose in brain parenchyma and cerebrospinal fluid of early Alzheimer’s disease detected by dynamic glucose-enhanced MRI

    Jianpan Huang, Peter C. M. van Zijl, Xiongqi Han, Celia M. Dong, Gerald W. Y. Cheng, Kai-Hei Tse, Linda Knutsson, Lin Chen, Joseph H. C. Lai, Ed X. Wu, Jiadi Xu, Kannie W. Y. Chan

    Download Supplement

    This PDF file includes:

    • Figs. S1 to S7

    Files in this Data Supplement:

Stay Connected to Science Advances

Navigate This Article