Linker-mediated self-assembly of mobile DNA-coated colloids

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Science Advances  20 May 2020:
Vol. 6, no. 21, eaaz6921
DOI: 10.1126/sciadv.aaz6921
  • Fig. 1 Linker-mediated mDNACCs.

    (A) Schematic representation of binary mNDACCs, in which the colloidal interaction is mediated via the bridging of free DNA linkers that bind with the mobile ssDNA receptors on mDNACCs. (B) A magnification of the yellow region in (A). Here, mobile ssDNA receptors (red or blue) coated on mDNACCs can bind with the ssDNA tails (red or blue spheres) of free linkers of the same color. (C) The linker-mediated effective interaction βUeff(r) as a function of the center-to-center distance between two mDNACCs r for different βμ with βΔGbind = −3.0, ni = 50, l = 0.2R, and rc = 0.01R. Solid lines are from Eq. 7, and symbols are from the direct MC simulations with explicit linkers.

  • Fig. 2 Re-entrant melting of linker-mediated mDNACCs.

    (A and B) βUeff(2R + 2rc) (A) and pib(2R+2rc) (B) as a function of βμ between two mDNACCs with ni = 50 for various βΔGbind. The solid lines are from theoretical prediction (Eq. 7), and the symbols are from direct simulations with explicit DNA linkers. (C) Typical snapshot for direct simulations of two linker-mediated mDNACCs with explicit DNA linkers with βΔGbind = −4.0 at βμ = −8.05, −1.90, and 2.0 (from top to bottom), where the (red and blue) big spheres and white line segments are mDNACCs and DNA linkers, respectively.

  • Fig. 3 Simulation of re-entrant melting.

    (A) Singlet fraction in binary linker-mediated mDNACCs (packing fraction η = 0.1) as a function of βμ for different βΔGbind (with lines as a guide to the eye). (B to D) The typical snapshots of the system at βμ = −6.5 (B), −2.5 (C), and 2.5 (D), where nonsinglets are drawn 10 times smaller. Here, ni = 50 and βΔGbind = −4.0 at the packing fraction η = 0.1.

  • Fig. 4 Entropy-driven linker-mediated mDNACCs at the strong binding limit.

    (A) Effective pair interaction βUeff(2R + 2rc) as a function of βΔGbind for various βμ predicted by Eqs. 7 (solid lines) and 11 (dashed lines). (B) Singlet fraction in binary linker-mediated mDNACCs at η = 0.1 as a function of βΔGbind for different βμ (with lines as a guide to the eye). Here, ni = 50. (C) Effective pair interaction βUeff(2R+2rc) as a function of βμ for various ni at βΔGbind → −∞ (Eq. 11).

  • Fig. 5 Crystallization of linker-mediated mDNACCs.

    EOS of the linker-mediated mDNACC systems at βμ = 1.6 with different βΔGbind, where the filled and open symbols are the results from NPT simulations expanding from perfect CsCl crystals and compressing from random fluids, respectively. The insets are the snapshots of fluid and nucleated CsCl crystal, respectively.

Supplementary Materials

  • Supplementary Materials

    Linker-mediated self-assembly of mobile DNA-coated colloids

    Xiuyang Xia, Hao Hu, Massimo Pica Ciamarra, Ran Ni

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