Research ArticleMATERIALS SCIENCE

Charge-reversal surfactant antibiotic material for reducing microbial corrosion in petroleum exploitation and transportation

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Science Advances  19 Jun 2020:
Vol. 6, no. 25, eaba7524
DOI: 10.1126/sciadv.aba7524
  • Fig. 1 Charge-reversal and antibacterial function of C12N-DCA.

    (A) Charge-reversal reaction of C12N-DCA. (B) Schematic representation of the antibacterial process of charge-reversal antibacterial agent during petroleum extraction.

  • Fig. 2 The evaluation of CCK-8 assay.

    (A) Correlation between average absorbance at 450 nm and relative content of D. desulfuricans after incubation at 37°C for 30 min with CCK-8. (B) Mean relative SD of different relative content of D. desulfuricans. The concentration of cultured D. desulfuricans was about 103 per milliliter as indicated by QuickChek SRB detecting result (fig. S2). The adding amount of CCK-8 was 1:10 (v:v).

  • Fig. 3 The antibacterial activities and mechanism of CnN-DCA.

    (A) Relative content of D. desulfuricans after being anaerobically incubated at 37°C for 3 days with C12N-DCA, C12N+ (positive control), and C12N-CCA (negative control). Incipient pH 6, n = 12. (B) Relative content of D. desulfuricans after being anaerobically incubated at 37°C for 3 days with C12N-DCA at incipient pH 6, 7, and 8. n = 12. (C) Hydrolysis reaction and hydrolysis kinetics of C4N-DCA at pH 6, 7, and 8 (pH buffer) at 20°C. (D) Relative content of D. desulfuricans after being anaerobically incubated at 37°C for 3 days with C8N-DCA, C10N-DCA, C12N-DCA, and C14N-DCA. Incipient pH 6, n = 12. All relative cell viabilities were measured by CCK-8 assay on the basis of the method established above. Data represent means ± SE.

  • Fig. 4 The antibacterial activity of C12N-DCA in the presence of petroleum and montmorillonite.

    (A) Photos of culture systems after being anaerobically incubated at 37°C for 3 days with C12N-DCA in the presence of petroleum and montmorillonite. The upper layer in each vial was electric desalted petroleum. There was neither C12N-DCA nor D. desulfuricans in the BLANK group. The concentration referred to the concentration of C12N-DCA. Incipient pH 6. (B) Relative content of D. desulfuricans after being anaerobically incubated at 37°C for 3 days with C12N-DCA in the presence of petroleum and montmorillonite. Incipient pH 6, n = 3. All relative cell viabilities were measured by CCK-8 assay on the basis of the method established above. Data represent means ± SE. Photo credit: L.Z., Tsinghua University.

  • Fig. 5 The antibacterial activity and inhibiting effect on SRB corrosion of C12N-DCA after being adsorbed by montmorillonite.

    (A) Photos of culture systems after being anaerobically incubated at 37°C for 3 days (the first culture cycle). Incipient pH 6. The C12N-DCA and BZK experienced the adsorption of montmorillonite before being added, of which the concentrations were 300 μM if the antibiotics were not adsorbed by montmorillonite at all. There were neither antibiotics nor D. desulfuricans in the BLANK group. There were no antibiotics in the BACTERIA group. The blur of the photo was due to the antislip strip on the six-well plate. (B) Relative content of D. desulfuricans after being anaerobically incubated at 37°C for the first culture cycle. (C) Photos of culture systems after being anaerobically incubated at 37°C for another 3 days (the second culture cycle). Pieces of iron foils were put in each well before the second culture cycle. (D) Relative content of D. desulfuricans after being anaerobically incubated at 37°C for the second culture cycle. (E) Photos of iron foils before and after corrosion. The backgrounds were removed. (F) Mean corrosion rates of iron foils. All relative cell viabilities were measured by CCK-8 on the basis of the method established above. Data represent means ± SE, n = 3. Photo credit: L.Z., Tsinghua University.

Supplementary Materials

  • Supplementary Materials

    Charge-reversal surfactant antibiotic material for reducing microbial corrosion in petroleum exploitation and transportation

    Lingda Zeng, Yincheng Chang, Yukun Wu, Jinpeng Yang, Jiang-Fei Xu, Xi Zhang

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