Research ArticleAPPLIED SCIENCES AND ENGINEERING

Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome- and transcriptome-wide off-target effects

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Science Advances  15 Jul 2020:
Vol. 6, no. 29, eaba1773
DOI: 10.1126/sciadv.aba1773

Article Information

vol. 6 no. 29

Online ISSN: 
History: 
  • Received for publication November 14, 2019
  • Accepted for publication June 2, 2020
  • .

Author Information

  1. Sangsin Lee1,*,
  2. Ning Ding2,*,
  3. Yidi Sun3,4,
  4. Tanglong Yuan5,
  5. Jing Li1,
  6. Qichen Yuan2,
  7. Lizhong Liu6,
  8. Jie Yang2,
  9. Qian Wang7,
  10. Anatoly B. Kolomeisky2,7,8,
  11. Isaac B. Hilton1,6,
  12. Erwei Zuo5, and
  13. Xue Gao1,2,
  1. 1Department of Bioengineering, Rice University, Houston, TX 77030, USA.
  2. 2Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
  3. 3CAS Key Laboratory of Systems Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
  4. 4Bio-Med Big Data Center, Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
  5. 5Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.
  6. 6Department of Biosciences, Rice University, Houston, TX 77005, USA.
  7. 7Center for Theoretical and Biological Physics, Rice University, Houston, TX 77005, USA.
  8. 8Department of Chemistry, Rice University, Houston, TX 77005, USA.
  1. Corresponding author. Email: xue.gao{at}rice.edu (X.G.); erweizuo{at}163.com (E.Z.)
  • * These authors contributed equally to this work.

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