Research ArticleAPPLIED SCIENCES AND ENGINEERING
Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome- and transcriptome-wide off-target effects
- View ORCID ProfileSangsin Lee1,*,
- View ORCID ProfileNing Ding2,*,
- View ORCID ProfileYidi Sun3,4,
- View ORCID ProfileTanglong Yuan5,
- Jing Li1,
- Qichen Yuan2,
- View ORCID ProfileLizhong Liu6,
- View ORCID ProfileJie Yang2,
- View ORCID ProfileQian Wang7,
- View ORCID ProfileAnatoly B. Kolomeisky2,7,8,
- View ORCID ProfileIsaac B. Hilton1,6,
- View ORCID ProfileErwei Zuo5,† and
- View ORCID ProfileXue Gao1,2,†
- 1Department of Bioengineering, Rice University, Houston, TX 77030, USA.
- 2Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
- 3CAS Key Laboratory of Systems Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
- 4Bio-Med Big Data Center, Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
- 5Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.
- 6Department of Biosciences, Rice University, Houston, TX 77005, USA.
- 7Center for Theoretical and Biological Physics, Rice University, Houston, TX 77005, USA.
- 8Department of Chemistry, Rice University, Houston, TX 77005, USA.
- ↵†Corresponding author. Email: xue.gao{at}rice.edu (X.G.); erweizuo{at}163.com (E.Z.)
↵* These authors contributed equally to this work.
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Science Advances 15 Jul 2020:
Vol. 6, no. 29, eaba1773
DOI: 10.1126/sciadv.aba1773
Vol. 6, no. 29, eaba1773
DOI: 10.1126/sciadv.aba1773
Sangsin Lee
1Department of Bioengineering, Rice University, Houston, TX 77030, USA.
Ning Ding
2Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
Yidi Sun
3CAS Key Laboratory of Systems Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
4Bio-Med Big Data Center, Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Tanglong Yuan
5Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.
Jing Li
1Department of Bioengineering, Rice University, Houston, TX 77030, USA.
Qichen Yuan
2Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
Lizhong Liu
6Department of Biosciences, Rice University, Houston, TX 77005, USA.
Jie Yang
2Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
Qian Wang
7Center for Theoretical and Biological Physics, Rice University, Houston, TX 77005, USA.
Anatoly B. Kolomeisky
2Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
7Center for Theoretical and Biological Physics, Rice University, Houston, TX 77005, USA.
8Department of Chemistry, Rice University, Houston, TX 77005, USA.
Isaac B. Hilton
1Department of Bioengineering, Rice University, Houston, TX 77030, USA.
6Department of Biosciences, Rice University, Houston, TX 77005, USA.
Erwei Zuo
5Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.
Xue Gao
1Department of Bioengineering, Rice University, Houston, TX 77030, USA.
2Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, USA.
