October 2020
Vol 6, Issue 43

About The Cover

Cover image expansion

ONLINE COVER While fluorescence imaging is an essential tool for biologists and neuroscientists, conventional microscopes and miniscopes (used to image freely behaving animals) have been constrained by limited space-bandwidth product, shallow depth-of-field, and inability to resolve 3D distributed emitters. To overcome these limitations, Xue et al. developed a Computational Miniature Mesoscope (CM2). This light, compact mesoscope integrates both a microlens array for imaging and an LED array for excitation within the same platform, allowing the device to perform single-shot 3D imaging across an 8 × 7-mm2 field-of-view and 2.5-mm depth-of-field. The CM2 provides at least a 10 times field of view gain and a 100 times depth-of-field improvement over existing miniscopes. Xue et al. tested the device using volumetrically distributed fluorescent beads and fibers and used phantom experiments to measure the effects of bulk scattering and background fluorescence. The researchers suggest this mesoscope may be useful for a wide range of applications in biomedicine and 3D neural recording. [CREDIT: XUE ET AL./SCIENCE ADVANCES]